PMID: 12781979Jun 5, 2003Paper

cDNA cloning of a defender against apoptotic cell death 1 (DAD1) homologue, responsive to external temperature stimulus from the spider, Araneus ventricosus

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Kwang Sik LeeByung Rae Jin

Abstract

A cDNA encoding a defender against apoptotic cell death (DAD1) homologue was cloned from a cDNA library of the spider, A. ventricosus. Sequence analysis of the cDNA encoding the DAD1 homologue of A. ventricosus revealed that the 339-bp cDNA has an open reading frame of 113 amino acid residues. The deduced amino acid sequence of the A. ventricosus DAD1 homologue showed 75.4% of identity to Drosophila melanogaster, 74.6% of identity to Xenopus laevis, and 73.1% of identity to Homo sapiens, Sus scrofa, Mesocricetus auratus, Rattus norvegicus and Mus musculus. All animal DAD1 including A. ventricosus DAD1 homologue formed a subgroup, excluding all plant DAD1 proteins in the phylogenetic analysis. Northern blot analysis shows that the transcripts of A. ventricosus DAD1 homologue gene are present in all tissues examined, suggesting that A. ventricosus DAD1 is expressed in most, if not all, body tissues. Interestingly, the transcript levels of A. ventricosus DAD1 homologue gene were particularly high when exposed at low (4 degrees C) and high (37 degrees C) temperatures, suggesting that the gene is responsive to temperature stimuli.

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Citations

Mar 19, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Hu WanByung Rae Jin
Apr 23, 2014·The Kaohsiung Journal of Medical Sciences·Chien-Ming ChenChung-Lung Cho
Feb 6, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Omprakash Mittapalli, Richard H Shukle
Feb 8, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Ji Hee HanByung Rae Jin
Nov 26, 2008·Fish & Shellfish Immunology·M Prado-AlvarezA Figueras

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis