PMID: 8944654Nov 1, 1996Paper

cDNA cloning of a functional water channel from toad urinary bladder epithelium

The American Journal of Physiology
T MaA S Verkman

Abstract

A cDNA was cloned from the epithelium of toad (Bufo marinas) urinary bladder, based on homology to the mammalian aquaporins (AQP). The cDNA [947 base pairs (bp), identified as AQP-t1] encoded a 272-amino acid protein with 76% identity to mammalian aquaporin-1 (AQP-1) and 88% identity to frog water channel FA-CHIP. AQP-t1 cDNA was nearly identical to a fragment of a nonfunctional cDNA cloned recently from toad bladder ["AQP-TB"; J. Siner, A. Paredes, C. Hosselet, T. Hammond, K. Strange, and H.W. Harris, Am. J. Physiol. 270 (Cell Physiol. 39): C372-C381, 1996], except for reading frame shifts at bp 253, 264, and 682, two single amino acid deletions, a different 3'-coding sequence downstream from bp 786, and a different 5' sequence upstream from bp 9. Water permeability (Pf) in Xenopus laevis oocytes expressing AQP-t1 cRNA was strongly increased from (0.83 +/- 0.06) x 10(-3) cm/s (water-injected control) to (17 +/- 4) x 10(-3) cm/s, with 80% inhibition by 0.3 mM HgCl2; glycerol and urea permeabilities were not increased. Northern blot analysis showed a single AQP-t1 mRNA of 2.8 kb in eye > lung > urinary bladder > skin > stomach approximately heart, brain, and intestine. AQP-t1 mRNA expression was not changed by a 3-day dehydratio...Continue Reading

References

Dec 1, 1989·The Journal of General Physiology·L B Shi, A S Verkman
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·M A Knepper
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·M EchevarriaG Frindt
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·A FrigeriA S Verkman

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Citations

Nov 14, 1998·Journal of Hypertension·G HeinertD J Paterson
Jun 16, 1999·The Journal of Experimental Zoology·J P CostanzoR E Lee
Sep 16, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Sarah L ZimmermanCarissa M Krane
Jul 27, 2007·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Carissa M Krane, David L Goldstein
Sep 27, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Hiroko Nishimura, Yimu Yang
Jun 29, 2000·Annual Review of Biochemistry·M BorgniaP Agre
Jun 10, 2011·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Venkateshwar MutyamCarissa M Krane
Oct 16, 1999·Anesthesiology·W SteudelW M Zapol
Oct 16, 1999·Journal of Molecular Biology·M J BorgniaP Agre
Feb 14, 2006·General and Comparative Endocrinology·Minoru Uchiyama, Norifumi Konno
Feb 3, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Masakazu SuzukiShigeyasu Tanaka
Mar 10, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Masakazu Suzuki, Shigeyasu Tanaka

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