cDNA cloning of a novel rainbow trout SRY-type HMG box protein, rtSox23, and its functional analysis

Gene
A YamashitaT Shiba

Abstract

We have isolated a cDNA clone for a new member of Sox genes, termed rtSox23, from a rainbow trout ovary cDNA library. rtSox23 mRNA was notably expressed in ovary and brain. rtSox23 contains a leucine zipper in addition to an SRY-type HMG box. Although the recombinant HMG box region protein of rtSox23 could bind to an AACAAT sequence, the full-length rtSox23 could form a homodimer and did not bind to the sequence. Furthermore, using a two-hybrid system, we have isolated a cDNA clone encoding a protein that bound to the leucine zipper region of rtSox23. This protein was the rainbow trout homologue of mouse nucleoporin p62, which is a component of the nuclear pore complex in nuclear envelope. The rainbow trout p62 mRNA was also prominent in ovary and brain. Taken together, these results suggest that the rainbow trout p62 associates with rtSox23 in vivo and modulates the function of rtSox23.

References

May 9, 1991·Nature·P KoopmanR Lovell-Badge
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G Blobel
Jan 1, 1995·Advances in Genetics·A J Schafer
Nov 1, 1994·Journal of Structural Biology·N Panté, U Aebi
Jan 1, 1995·Annual Review of Biochemistry·L I Davis
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·D KraemerA Radu
Mar 1, 1994·Trends in Genetics : TIG·R GrosschedlJ Pagel
Apr 25, 1994·Nucleic Acids Research·V R HarleyP N Goodfellow
Dec 11, 1995·FEBS Letters·M ItoT Shiba
Mar 1, 1996·Biochimica Et Biophysica Acta·N KomatsuS Aiso
Apr 1, 1997·Molecular and Cellular Biology·V LefebvreB de Crombrugghe
May 1, 1997·European Journal of Immunology·M W SchilhamH C Clevers

❮ Previous
Next ❯

Citations

Dec 17, 2008·Molecular and Cellular Endocrinology·Laia Navarro-MartínFrancesc Piferrer
Jun 6, 2015·Molecular and Cellular Biochemistry·Mikella DaigleLuc J Martin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.