PMID: 8985118Dec 1, 1996Paper

cDNA cloning of a novel WD repeat protein mapping to the 9q22.3 chromosomal region

DNA and Cell Biology
P G Zaphiropoulos, R Toftgård

Abstract

To identify expressed sequences from the candidate genomic region of the nevoid basal cell carcinoma syndrome (9q22.3), the CpG island rescue PCR methodology was employed on the yeast artificial chromosome (YAC) ICI-8AD8 that contains microsatellite marker D9S180. A positive clone (IR10, size 350 bp) was isolated by screening a human epidermal cDNA library with the island rescue PCR products and mapped back to the 9q22.3 region. To obtain additional sequence information from IR10, the rapid amplification of cDNA ends (RACE) methodology was employed. Within the longest IR10 RACE product, two segments having similarities with coronin, a G-like and actin-binding protein of Dictyostelium discoideum, as well as with p57, a recently cloned human actin-binding protein, were revealed. In fact, these two segments have properties of typical exons, suggesting that the RACE products and clone IR10 represent unspliced pre-mRNAs. Using the RACE methodology with primers originating from these two exons, it was shown that they splice together and the sequences corresponding to the processed IR10 mRNA were revealed. This mRNA maps back to 9q22.3 and furthermore was found to code for a novel protein composed of 525 amino acids and containing fiv...Continue Reading

References

May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A J BucklerD E Housman
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M A ClarkJ S Bomalaski
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·S MunemitsuP Polakis
Jan 1, 1995·Human Molecular Genetics·S M ShanleyG Chenevix-Trench
Aug 1, 1994·Genomics·C WickingG Chenevix-Trench
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·J M ValdesF S Collins

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Citations

Oct 3, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·I VorechovskýR Toftgård
May 14, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Vasily Rybakin, Christoph S Clemen
Apr 9, 2005·The European Journal of Neuroscience·Andreas HasseChristoph S Clemen
Mar 22, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·S AsanoE Nishida
Nov 13, 1999·Nature Cell Biology·P KogermanP G Zaphiropoulos
Aug 26, 1999·Trends in Cell Biology·E L de Hostos

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