cDNA cloning of porcine granulocyte-macrophage colony-stimulating factor

Immunology and Cell Biology
S Inumaru, H Takamatsu

Abstract

Porcine granulocyte-macrophage colony-stimulating factor (GM-CSF) cDNA was cloned by using polymerase chain reaction (PCR) and sequenced. A coding sequence of the porcine GM-CSF precursor protein, including the signal peptide sequence and stop codon, is 435 bp in length. The identities of the porcine GM-CSF coding sequence when compared to ovine, bovine, human and murine sequences were 89, 86, 83 and 70% at the nucleotide level, and 80, 74, 73, and 56% at the amino acid level. The hydrophobicity profiles, putative glycosylation sites and positions of cysteine residues were highly conserved in porcine, ovine, bovine and human GM-CSF but not murine. This is the first report of the porcine GM-CSF cDNA cloning and sequence.

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Citations

Apr 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Q Gao, J E Womack
Apr 6, 2004·Developmental and Comparative Immunology·Daniel R BarredaMiodrag Belosevic
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Feb 5, 2013·Insect Biochemistry and Molecular Biology·Satoko WatanabeShigeki Inumaru
Oct 15, 2003·FEMS Immunology and Medical Microbiology·Young Wook ChoHan Sang Yoo

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