cDNA cloning of two gonadotropin beta subunits (GTH-Ibeta and -IIbeta) and their expression profiles during gametogenesis in the Japanese flounder (Paralichthys olivaceus)

General and Comparative Endocrinology
S KajimuraK Aida

Abstract

To clarify the profiles of two distinct gonadotropin (GTH-I and -II) mRNA levels during gametogenesis in a multiple spawner, the Japanese flounder (Paralichthys olivaceus), the cDNAs encoding GTH-Ibeta and -IIbeta from the pituitary gland have been cloned and sequenced. The nucleotide sequence of GTH-Ibeta was 542 bp long, encoding 120 amino acids, and that of GTH-IIbeta was 554 bp long, encoding 145 amino acids. In females, Northern blot analysis has revealed that relative mRNA levels of GTH-Ibeta and -IIbeta were low in immature fish, showed a gradual increase with ovarian development, and reached the highest level at the maturation stage. Both GTH-Ibeta and -IIbeta mRNA levels were highly correlated with gonadosomatic index (GSI) values and with circulating estradiol-17beta and testosterone (T) levels. In males, the mRNA levels of GTH-Ibeta increased with the increase in GSI values and in circulating 11-ketotestosterone and T levels, whereas the mRNA levels of GTH-IIbeta did not show any correlation with GSI values and with circulating steroid levels, suggesting a difference in regulatory mechanisms of GTH-I and -II synthesis in males. The similar changes in GTH-Ibeta and -IIbeta mRNA levels during oogenesis are considered t...Continue Reading

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Citations

Feb 6, 2008·Zebrafish·Andrew C Wong, Alison L Van Eenennaam
Mar 26, 2002·Molecular and Cellular Endocrinology·D L Huggard-NelsonH R Habibi
Aug 25, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Masafumi AmanoKunio Yamamori
Jun 4, 2015·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Bao ShiKun Zang
May 15, 2009·Environmental Health Perspectives·Daniel L VilleneuveGerald T Ankley
Jun 1, 2005·General and Comparative Endocrinology·Abhijit ChatterjeeJohn Yuh-Lin Yu
Nov 3, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Michela CandelmaOliana Carnevali
Apr 7, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Christian MittelholzerBirgitta Norberg
May 5, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Finn-Arne WeltzienBirgitta Norberg
Aug 15, 2021·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Yo YamaguchiTakashi Todo
Aug 19, 2009·General and Comparative Endocrinology·B Levavi-SivanJ J Lareyre

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