PMID: 2112252Jun 1, 1990Paper

cDNA cloning, sequence, and expression of carbonic anhydrase in Chlamydomonas reinhardtii: regulation by environmental CO2 concentration

Proceedings of the National Academy of Sciences of the United States of America
Hideya FukuzawaS Miyachi

Abstract

cDNA clones for the periplasmic carbonic anhydrase (CA; carbonate hydro-lyase, EC 4.2.1.1) of Chlamydomonas reinhardtii cells were isolated and characterized. The fact that the cloned cDNA fragments encoded a 377-amino acid polypeptide (41,626 Da) consisting of an NH2-terminal hydrophobic signal peptide of 20 amino acids, a large (35,603 Da) subunit polypeptide, and a small (4144 Da) subunit polypeptide indicates that the two subunits are cotranslated as a precursor polypeptide. The amino acid sequence of mature subunits deduced from the nucleotide sequence showed 20-22% homology with human CA isozymes (CAI, CAII, and CAIII). Three zinc-liganded histidine residues and those forming a hydrogen-bond network to zinc-bound solvent molecules were highly conserved in the plant and animal enzymes. Three possible asparagine-linked glycosylation sites were found in the large subunit. Northern blot analysis was carried out using the cDNA fragment as a probe. The level of 2.0-kilobase CA mRNA increased within 1 hr when CO2 concentration of the bubbling gas was changed from 5% to 0.04% (air level) in the presence of light. On the other hand, CA mRNA did not accumulate when CO2 concentration was lowered in the dark. Experiments using 3-(3,4...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 1, 1989·Molecular & General Genetics : MGG·E L de HostosA R Grossman
Jun 1, 1989·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R E Tashian
Aug 1, 1986·European Journal of Biochemistry·T ToguriS Miyachi
Mar 11, 1987·Nucleic Acids Research·J H BarlowP H Butterworth
Jun 11, 1987·Nucleic Acids Research·J C MontgomeryD Hewett-Emmett
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Jan 1, 1981·Annual Review of Biochemistry·S C Hubbard, R J Ivatt

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Dec 18, 2004·Plant Molecular Biology·Gustavo ParisiEduardo Zabaleta
Jan 19, 2011·Photosynthesis Research·Masato BabaYoshihiro Shiraiwa
Jan 9, 1992·Biochimica Et Biophysica Acta·L G MårtenssonU Carlsson
Jan 1, 1997·Pharmacology & Therapeutics·S Lindskog
Nov 26, 2002·Biochimica Et Biophysica Acta·Laura C ChiricaSven Lindskog
May 10, 2002·The Plant Journal : for Cell and Molecular Biology·Chung Soon Im, Arthur R Grossman
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S FujiwaraS Miyachi
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H FukuzawaS Miyachi
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·B E Alber, J G Ferry
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·M ErikssonG Samuelsson
May 4, 2004·Plant Physiology·Mautusi MitraJames V Moroney
Nov 1, 1990·Plant Physiology·M L Dionisio-SeseS Miyachi
Sep 9, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Kaoru SuzukiShi Yuan Yang
Sep 11, 1990·European Journal of Biochemistry·T KamoS Miyachi
Jun 15, 2007·Eukaryotic Cell·James V Moroney, Ruby A Ynalvez
Apr 17, 2007·Eukaryotic Cell·Heidi K LedfordKrishna K Niyogi
Nov 14, 2000·Journal of Bacteriology·K S SmithJ G Ferry
May 3, 2005·Annual Review of Plant Biology·Mario GiordanoJohn A Raven
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·K S SmithJ G Ferry
Apr 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Eric SoupeneSydney Kustu
Apr 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·H FukuzawaK Ohyama

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