Abstract
The complete CDS sequences of three porcine genes: UCHL3, RIT1 and CCND3 were amplified using RT-PCR based on the sequence information of the mouse or other mammals and referenced highly homologous pig ESTs. Sequence analysis of these three genes revealed that the porcine UCHL3 gene encodes a protein of 230 amino acids and has high homology with the ubiquitin carboxyl-terminal hydrolase isozyme L3 (UCHL3) of four species-bovine (97%), human (96%), mouse (95%) and rat (94%). The porcine RIT1 gene encodes a protein of 219 amino acids and has high homology with the GTP-binding protein Rit1 (RIT1) of two species-human (97%), mouse (97%). The porcine CCND3 gene encodes a protein of 292 amino acids and has high homology with the G1/S-specific cyclin-D3 (CCND3) of four species-bovine (98%), human (97%), mouse (93%) and rat (92%). The phylogenetic tree analysis revealed that the swine UCHL3 has a closer genetic relationship with the UCHL3 of bovine, and the swine RIT1 has closer genetic relationships with the RIT1 of human, but the swine CCND3 has a closer genetic relationship with the CCND3 of bovine. The RT-PCR gene expression analysis indicated that the swine UCHL3, RIT1 and CCND3 genes were differentially expressed in tissues inclu...Continue Reading
References
Jul 1, 1992·Genomics·T InabaA T Look
Nov 3, 1989·Science·K D WilkinsonJ Pohl
Oct 29, 1998·Biochemical and Biophysical Research Communications·H WadaT Kamitani
May 9, 2001·Biochemical and Biophysical Research Communications·Y OsawaK Wada
May 23, 2003·Human Molecular Genetics·Ekaterina SemenovaShirley M Tilghman
Nov 5, 2003·Proteomics·Myeong J NamSamir M Hanash
Nov 19, 2003·PLoS Biology·Ross C Hardison
Jan 6, 2005·Molecular and Cellular Biology·Geng-Xian Shi, Douglas A Andres
Jan 26, 2005·British Journal of Haematology·Matthew L SmithJude Fitzgibbon
Apr 16, 2005·The Journal of Biological Chemistry·Mitsunobu HoshinoShun Nakamura
May 11, 2005·Hippocampus·Marcelo A WoodTed Abel
Jun 28, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Hideaki TanamiJohji Inazawa
Dec 3, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yumiko KasugaiMasao Seto
Apr 4, 2006·Nature Immunology·A Byron CooperIannis Aifantis
Jul 4, 2006·The American Journal of Pathology·Yae SanoKeiji Wada
Dec 26, 2006·Molecular Biology Reports·G Y Liu, Y Z Xiong
Citations
May 9, 2012·Endocrinology·Wei ShenJulang Li
Feb 26, 2010·Molecular Biology Reports·H Y Chung, J Y Kim
May 31, 2018·Current Eye Research·Sajad Mir, Douglas A Andres
Sep 17, 2009·Molecular Biology Reports·Liu Yonggang