cDNA cloning, sequencing and chromosomal assignment of the gene for mouse complement factor I (C3b/C4b inactivator): identification of a species specific divergent segment in factor I

Molecular Immunology
J O MintaG Goldberger

Abstract

Factor I is an essential regulatory serine proteinase of the complement cascade. It cleaves and inactivates the C3b and C4b constituents of the C3 and C5 convertases and thereby regulates many complement-mediated activities. The human protein is a heterodimer composed of a 50 kDa non-catalytic subunit (which contains several domains, i.e. FIM, CD5, LDLr type A) disulfide linked to a 38 kDa catalytic subunit. Recent characterization of Xenopus factor I cDNA revealed a 29 residue negatively charged region in its heavy chain which is absent in the human protein (Kunnath-Muglia et al., Molec. Immun. 30, 1249-1256, 1993). We report the complete cDNA sequence of mouse factor I as well as a partial chicken factor I cDNA sequence. Alignment of these two sequences with the published sequences for human and Xenopus proteins (a) demonstrates an overall conservation of primary structure and domain organization of mouse factor I, and (b) defines a divergent segment (D segment) in each species. In Xenopus protein, the D segment includes the 29 residue negatively charged region. In each of the four species examined, the D segment differed in length, sequence, organization, and number of repeated subregions. These differences reflect a conside...Continue Reading

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Citations

Mar 22, 2002·Developmental and Comparative Immunology·Tokio TeradoHiroshi Kimura
Jun 24, 2003·Developmental and Comparative Immunology·Miki NakaoTomoki Yano
Feb 21, 2002·The International Journal of Biochemistry & Cell Biology·Jonas Heilskov GraversenSøren K Moestrup
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·A FortinP Gros
Jul 8, 2015·Developmental and Comparative Immunology·Jinsong XiangZhenxia Sha
Jun 12, 2002·Genes and Immunity·A FortinP Gros
May 27, 2005·Journal of the American Society of Nephrology : JASN·David KavanaghTimothy H J Goodship
May 10, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·L Lo Muzio
Feb 19, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Gerald SchlafOtto Götze
Jun 23, 2009·Fish & Shellfish Immunology·Jason W AbernathyZhanjiang Liu
Sep 28, 2010·Molecular Immunology·Laura A Fraczek, Brian K Martin

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