PMID: 9165083Mar 1, 1997Paper

cDNA cloning, tissue distribution, and subcellular localization of horseshoe crab big defensin

Biological Chemistry
S KawabataS Iwanaga

Abstract

A full-length cDNA for horseshoe crab big defensin with a strong antimicrobial activity was obtained from a hemocyte cDNA library. The open reading frame of the cDNA coded for an NH2-terminal signal sequence followed by a propeptide and the mature big defensin. The propeptide is linked to the mature protein through an -Arg-X-Lys/Arg-Arg- motif, the processing site for Kex2-like proteases. Northern blot analysis revealed that big defensin is expressed in all the tissues tested, suggesting that big defensin plays an important role not only in hemocytes but also in other tissues for host defense. The subcellular localization, determined by immunocytochemistry at ultrastructural level, confirmed the previous findings obtained by biochemical analysis that big defensin locates in both small and large granules in hemocytes. Big defensin is the first example to demonstrate the existence of broad tissue distribution in horseshoe crab.

Citations

Jan 16, 2002·Current Opinion in Immunology·Sadaaki Iwanaga
Jul 6, 2001·Insect Biochemistry and Molecular Biology·R JohnsW L Hynes
Jan 28, 2012·Fish & Shellfish Immunology·Lei TengShicui Zhang
Dec 16, 2011·Immunological Reviews·Robert I Lehrer, Wuyuan Lu
Apr 14, 2005·Journal of Biochemistry and Molecular Biology·Sadaaki Iwanaga, Bok Luel Lee
Oct 15, 2013·International Journal of Medical Microbiology : IJMM·Miriam Wilmes, Hans-Georg Sahl
Jun 18, 2004·Immunological Reviews·Kei-ichiro InamoriShun-ichiro Kawabata
Sep 17, 2004·Nature Reviews. Microbiology·Robert I Lehrer
Jan 6, 2010·International Journal of Environmental Research and Public Health·Dragan R MilićevićSrdan M Stefanović
May 20, 2020·Frontiers in Immunology·Marco GerdolDelphine Destoumieux-Garzón

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