cDNA microarray analysis of serially sampled cervical cancer specimens from patients treated with thermochemoradiotherapy

International Journal of Radiation Oncology, Biology, Physics
Erling Dahl BorkamoOlav Mella

Abstract

To elucidate changes in gene expression after treatment with regional thermochemoradiotherapy in locally advanced squamous cell cervical cancer. Tru-Cut biopsy specimens were serially collected from 16 patients. Microarray gene expression levels before and 24 h after the first and second trimodality treatment sessions were compared. Pathway and network analyses were conducted by use of Ingenuity Pathways Analysis (IPA; Ingenuity Systems, Redwood City, CA). Single gene expressions were analyzed by quantitative real-time reverse transcription-polymerase chain reaction. We detected 53 annotated genes that were differentially expressed after trimodality treatment. Central in the three top networks detected by IPA were interferon alfa, interferon beta, and interferon gamma receptor; nuclear factor kappaB; and tumor necrosis factor, respectively. These genes encode proteins that are important in regulation cell signaling, proliferation, gene expression, and immune stimulation. Biological processes over-represented among the 53 genes were fibrosis, tumorigenesis, and immune response. Microarrays showed minor changes in gene expression after thermochemoradiotherapy in locally advanced cervical cancer. We detected 53 differentially expr...Continue Reading

References

Jul 1, 1995·The Journal of Experimental Medicine·A L AngiolilloG Tosato
Oct 1, 1996·Genome Research·C A HeidP M Williams
Oct 7, 1997·The Journal of Experimental Medicine·M A Degli-EspostiC A Smith
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Sep 1, 2004·International Journal of Radiation Oncology, Biology, Physics·Yoko HarimaToyomasa Katagiri
May 17, 2007·Current Medicinal Chemistry·S G MaherA M Gamero
Jul 10, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Bradley J MonkWui-Jin Koh
Mar 4, 2008·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Erling Dahl BorkamoOlav Dahl
Apr 15, 2008·International Journal of Radiation Oncology, Biology, Physics·Ann H KloppThomas A Buchholz
Jul 9, 2008·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Erling Dahl BorkamoOystein Fluge
Nov 8, 2008·International Journal of Radiation Oncology, Biology, Physics·Martine FranckenaJacoba van der Zee
Nov 22, 2008·Cancer Detection and Prevention·Berenice Illades-AguiarMarco-Antonio Leyva-Vázquez
Dec 10, 2008·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Edwin van der WalGerard C van Rhoon
Feb 17, 2009·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Erling Dahl BorkamoØystein Fluge

❮ Previous
Next ❯

Citations

Feb 5, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jen-Tsan ChiMark W Dewhirst
Oct 23, 2013·Gynecologic Oncology·Yoo-Young LeeByoung-Gie Kim
Mar 21, 2020·Advanced Healthcare Materials·Ali FarzinAli Tamayol

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.