PMID: 11309148Apr 20, 2001Paper

cDNA microarray analysis of small plant tissue samples using a cDNA tag target amplification protocol

The Plant Journal : for Cell and Molecular Biology
M HertzbergJ Lundeberg

Abstract

Microarray technology is becoming an important comprehensive tool to study gene expression in plants. However, the use of this technology is limited by the large amount of sample tissue needed for microarray analysis. Generally, 50-200 microg of total RNA and 1-2 microg of mRNA is required for each hybridisation, which is equivalent to 50-100 mg of plant tissue. This requirement for large amounts of starting material severely constrains the use of microarrays for transcript profiling in specific tissues and cell types during plant development. Here we report on a robust and reliable target amplification method that enables transcript profiling from sub-mg amounts of plant tissue. Using 0.1 microg of total RNA we show that twofold expression differences are possible to distinguish with 99% confidence. We also demonstrate the application of this method in an analysis of secondary phloem development in hybrid aspen using defined tissue sections, corresponding to 2-4 cell layers with a fresh weight of approximately 0.5 mg.

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Citations

Nov 16, 2001·Phytochemical Analysis : PCA
Aug 4, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Sabine LezarB D Wingfield
Apr 12, 2002·Trends in Biotechnology·Farid E Ahmed
May 31, 2002·Gene·Charlotta AgatonJoakim Lundeberg
Jun 13, 2003·Gene·Tove AnderssonJacob Odeberg
May 7, 2003·Current Opinion in Biotechnology·Rishikesh BhaleraoGoran Sandberg
Sep 16, 2003·Current Opinion in Plant Biology·Tong Zhu
Jul 10, 2004·Molecular Plant-microbe Interactions : MPMI·Torben GjettingMichael F Lyngkjaer
Sep 12, 2002·Annual Review of Plant Biology·Zheng-Hua Ye
Aug 30, 2005·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Esther Edlundh-RoseJoakim Lundeberg
Apr 19, 2005·BMC Neuroscience·Maria SievertzonJoakim Lundeberg
Nov 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·M HertzbergG Sandberg
Apr 27, 2002·Physiologia Plantarum·Nigel ChaffeyBjörn Sundberg
Mar 1, 2011·Phytochemistry·Nieves AbrilJesús V Jorrin-Novo
Oct 23, 2015·Trends in Plant Science·Viktoriya AvramovaGerrit T S Beemster
May 13, 2008·Comptes rendus biologies·Sylvie Dinant
Dec 13, 2006·Plant Biotechnology Journal·Malcolm M CampbellSteve H Strauss
Sep 17, 2003·The Plant Journal : for Cell and Molecular Biology·F VilaineS Dinant
Jul 16, 2002·Annual Review of Biomedical Engineering·Michael J Heller
Mar 28, 2002·Environmental Pollution·Takashi MatsuyamaHikaru Saji
May 19, 2006·Expert Review of Molecular Diagnostics·Maria SievertzonJoakim Lundeberg
Jul 14, 2012·Microbes and Environments·Yong WangTakeshi Fujii
Oct 1, 2004·The New Phytologist·Gilles PilateJean-Charles Leplé

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