PMID: 15379434Sep 24, 2004Paper

cDNA of canine heat shock protein 70 (HSP70)

Veterinary Research Communications
Rui KanoA Hasegawa

Abstract

Full-length canine HSP70 cDNA was sequenced and the expression of HSP70 mRNA was investigated. The full-length cDNA sequence of the HSP70 gene (2322 bp) contained a single long open reading frame (1920 bp) coding a protein of 640 amino acids. The amino acid sequence of the canine HSP70 gene shared about 90-95% sequence similarity with bovine, human and mouse HSP70 proteins. Southern blot analysis with HSP70 probe gave three distinct bands of 9.4 kb, 5 kb and 4.4 kb in BamHI digests and two distinct bands of 19 kb and 4 kb in EcoRI digests. Canine HSP70 mRNA was detectable in canine peripheral blood mononuclear cells and stomach but not in liver, kidney, spleen, small intestine, large intestine and skin of dogs.

Citations

Mar 13, 2008·Cell Stress & Chaperones·Mariarita RomanucciLeonardo Della Salda
Aug 17, 2011·International Journal of Molecular Sciences·Mohamed S ElrobhMohammad D Bazzi
Jan 1, 2010·Molecular Biology International·Nitin GadeMohini Saini

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