CdTe nanocrystal-based electrochemical biosensor for the recognition of neutravidin by anodic stripping voltammetry at electrodeposited bismuth film

Biosensors & Bioelectronics
Dan DuXi Chen

Abstract

CdTe quantum dots (QDs)-based electrochemical sensor for recognition of neutravidin, as a model protein, using anodic stripping voltammetry at electrodeposited bismuth film is presented. This biosensor involves the immobilization of the captured QDs conjugates which was dissolved with 1M HCl solution to release cadmium ions and metal components were quantified by anodic stripping voltammetry after a 3-min accumulation at -1.2V on bismuth-film electrode (BiFE) of the biotin, served as recognition element, onto the gold surface in connection with a cysteamine self-assembled monolayer. The modification procedure was characterized by electrochemical impedance spectroscopy and atomic force microscopy. We exploit QDs as labels for amplifying signal output and monitoring the extent of competition process between CdTe-labeled neutravidin and the target neutravidin for the limited binding sites on biotin. As expected for the competitive mechanism, the recognition event thus yields distinct cadmium stripping voltammetric current peak, whose response decreases upon increasing the level of target neutravidin concentrations. Under optimal conditions, the voltammetric response is highly linear over the range of 0.5-100 ngL(-1) neutravidin an...Continue Reading

References

Jan 12, 2008·Chemical Communications : Chem Comm·Cunwang GeHuangxian Ju

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Citations

Apr 14, 2017·The Journal of Physical Chemistry. a·Andrew J S ValentineDavid A Mazziotti
Sep 30, 2010·Journal of Biophotonics·Ken-Tye YongParas N Prasad
Oct 25, 2011·The Analyst·Qiang Ma, Xingguang Su
Jul 6, 2012·Chemical Society Reviews·Matteo AmeliaAlberto Credi
Mar 16, 2021·Emergent Materials·Sultan ŞahinLevent Trabzon

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