CDX2 protein expression compared to alcian blue staining in the evaluation of esophageal intestinal metaplasia

World Journal of Gastroenterology : WJG
Dennis R JohnsonDomenico Coppola

Abstract

To compare the sensitivity and specificity of CDX2 and alcian blue (AB) pH 2.5 staining in identifying esophageal intestinal metaplasia. One hundred and ninty-nine biopsies from 186 patients were retrospectively reviewed and categorized as Barrett's esophagus (BE) (n = 108); non-Barrett's esophagus (NBE) (n = 48); columnar blue cells (CB) and esophageal glands (EG) (n = 43). The biopsies were stained with AB and immunostained for CDX2 using a mouse monoclonal antibody from Biogenex (clone CDX2-88) and the Ventana Discovery X automated immunostainer. The positive and negative predictive value of each group was used to determine the predictive power of CDX2 and AB in diagnosing intestinal metaplasia. All of the 108 BE biopsies (100%) were positive for AB and 102 of them (94.4%) were positive for CDX2. The six BE patients (5.6%) who failed to stain with CDX2 were found to have lost the focus of intestinal metaplasia upon deeper sectioning for immunostaining. Both AB and CDX2 were negative in 43 out of 48 (89.6%) NBE cases. Five NBE patients (10.4%) were falsely positive for AB due to the presence of EG and CB in these biopsies. These cases were all CDX2 negative. In addition, 5 AB negative NBE were found to be CDX2 positive. Based...Continue Reading

References

Nov 1, 1994·Molecular and Cellular Biology·E SuhP G Traber
Feb 1, 1996·Molecular and Cellular Biology·E Suh, P G Traber
Nov 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·F BeckR James
Oct 16, 1999·The American Journal of Gastroenterology·J D Long, R C Orlando
Oct 21, 2000·Gastroenterology·D G SilbergP G Traber
Jan 28, 2003·The American Journal of Surgical Pathology·M BarbareschiC Doglioni
Feb 1, 2003·Journal of Gastroenterology·Akashi EdaKentaro Sugano
Sep 19, 2003·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Christopher A MoskalukHenry F Frierson
Oct 25, 2003·The American Journal of Surgical Pathology·Roy W PhillipsChristopher A Moskaluk
Nov 15, 2003·Journal of Cancer Research and Clinical Oncology·Tsutomu MizoshitaMasae Tatematsu
May 29, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Gabriel M GroismanAlona Meir
Mar 18, 2008·The American Journal of Gastroenterology·Kenneth K WangUNKNOWN Practice Parameters Committee of the American College of Gastroenterology
Apr 14, 2009·The American Journal of Surgical Pathology·Hejin P HahnRobert D Odze
Dec 23, 2011·Development·Emma J StringerFelix Beck
Mar 19, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal

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Citations

Apr 21, 2016·The American Journal of Surgical Pathology·Nicole C Panarelli, Rhonda K Yantiss
Feb 26, 2016·World Journal of Gastrointestinal Pathophysiology·Gen KusakaTooru Shimosegawa
Jul 6, 2021·Frontiers in Oncology·Lu ZhangMuhan Lü

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