C/EBP-beta drives expression of the nutritionally regulated promoter IA of the acetyl-CoA carboxylase-alpha gene in cattle

Biochimica Et Biophysica Acta
Xuanming ShiHans-Martin Seyfert

Abstract

Acetyl-CoA carboxylase-alpha (ACC-alpha) is the rate-limiting enzyme for de novo fatty acid synthesis. Among the four promoters expressing the bovine gene, promoter IA (PIA) is dominantly active and nutritionally regulated in lipogenic tissues. CCAAT/enhancer binding proteins are crucially involved in regulating the activity of this promoter. We examine here, which member of this family of transcription factors is most important for promoter activation. To differentiate the individual contribution of different members of the C/EBP family transcription factors controlling the ACC-alpha gene expression in cattle, we established vectors expressing full length (FL) or N-terminally truncated (DeltaN) variants of the C/EBP factors (alpha, -beta, -delta, and -epsilon) in mammalian cells. Using nuclear extracts of cells expressing the DeltaN-C/EBP factors we determined in electrophoretic mobility shift assays that C/EBPalpha, -beta and -epsilon, but not C/EBPdelta may directly bind to the cognate C/EBP-binding site in the immediate ACC-alpha PIA. Co-transfection analyses of the various FL-C/EBP expression vectors together with a reporter gene driven by the ACC-alpha-PIA promoter demonstrated that C/EBPbeta has the strongest activation ...Continue Reading

References

Oct 1, 1996·Journal of Animal Science·Y Taniguchi, Y Sasaki
Feb 1, 1997·Journal of Animal Science·Y Taniguchi, Y Sasaki
Feb 1, 1997·Journal of Animal Science·I YamaokaY Sasaki
Jan 1, 1997·Annual Review of Nutrition·K H Kim
Feb 5, 1998·Biochemical Society Transactions·M T Travers, M C Barber
May 8, 2001·The Journal of Biological Chemistry·B L O'CallaghanH C Towle
May 15, 2002·The Biochemical Journal·Dipak P Ramji, Pelagia Foka
Mar 8, 2005·Biochimica Et Biophysica Acta·Michael C BarberMaureen T Travers
Sep 27, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Wolfgang Tomek, Tatjana Smiljakovic
Nov 16, 2006·Physiological Genomics·Michael C RudolphMargaret C Neville
Apr 28, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shaikh Mizanoor RahmanJacob E Friedman
Dec 3, 2008·Journal of Lipid Research·Salih J Wakil, Lutfi A Abu-Elheiga

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Citations

Sep 4, 2010·Developmental and Comparative Immunology·Alexander ReblHans-Martin Seyfert
Oct 26, 2016·Developmental and Comparative Immunology·Alexander ReblHans-Martin Seyfert
Jun 22, 2012·Molecular Biology Reports·Hirokazu MatsumotoHideyuki Mannen
Jan 1, 2021·Bioinformatics·Salvador Casaní-GaldónAna Conesa

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