C/EBPβ (CEBPB) protein binding to the C/EBP|CRE DNA 8-mer TTGC|GTCA is inhibited by 5hmC and enhanced by 5mC, 5fC, and 5caC in the CG dinucleotide

Biochimica Et Biophysica Acta
Syed K SayeedCharles Vinson

Abstract

During mammalian development, some methylated cytosines (5mC) in CG dinucleotides are iteratively oxidized by TET dioxygenases to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC). The effect of these cytosine oxidative products on the sequence-specific DNA binding of transcription factors is being actively investigated. Here, we used the electrophoretic mobility shift assay (EMSA) to examine C/EBPα and C/EBPβ homodimers binding to all 25 chemical forms of a CG dinucleotide for two DNA sequences: the canonical C/EBP 8-mer TTGC|GCAA and the chimeric C/EBP|CRE 8-mer TTGC|GTCA. 5hmC in the CG dinucleotide in the C/EBP|CRE motif 8-mer TGAC|GCAA inhibits binding of C/EBPβ but not C/EBPα. Binding was increased by 5mC, 5fC and 5caC. Circular dichroism monitored thermal denaturations for C/EBPβ bound to the C/EBP|CRE motif confirmed the EMSA. The structural differences between C/EBPα and C/EBPβ that may account for the difference in binding 5hmC in the 8-mer TGAC|GCAA are explored.

References

Apr 1, 1993·Current Opinion in Genetics & Development·P H Tate, A P Bird
Nov 7, 1998·The Journal of Biological Chemistry·G J DarlingtonO A MacDougald
May 15, 2002·The Biochemical Journal·Dipak P Ramji, Pelagia Foka
Aug 23, 2002·Molecular and Cellular Biology·Charles VinsonMaria Bonovich
Jul 17, 2004·Genome Research·Peter C FitzGeraldCharles Vinson
Mar 31, 2009·Future Microbiology·Yujie LiuBrian Wigdahl
Apr 18, 2009·Science·Skirmantas Kriaucionis, Nathaniel Heintz
May 18, 2011·Genes & Development·Aimée M Deaton, Adrian Bird
Jun 28, 2011·Biochemical and Biophysical Research Communications·Julia RobertsonArne Klungland
Mar 6, 2012·Biochimica Et Biophysica Acta·Raghunath Chatterjee, Charles Vinson
Dec 19, 2012·Epigenomics·Charles Vinson, Raghunath Chatterjee
Feb 26, 2013·Cell·Cornelia G SpruijtMichiel Vermeulen
May 24, 2013·Nature Reviews. Molecular Cell Biology·William A PastorAnjana Rao
Sep 21, 2013·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Jean Cadet, J Richard Wagner
Nov 30, 2013·Chemical Communications : Chem Comm·Lukas LercherChristopher J Schofield
Dec 29, 2013·Nucleic Acids Research·Montse GustemsWolfgang Hammerschmidt
Dec 29, 2013·Stem Cell Reports·Jamie A HackettM Azim Surani
May 13, 2014·Cell and Tissue Research·Gerd P PfeiferSeung-Gi Jin
Jul 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ageliki TsagaratouAnjana Rao

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Citations

Aug 4, 2016·Integrative Biology : Quantitative Biosciences From Nano to Macro·Syed Khund-SayeedCharles Vinson
Dec 3, 2016·Nucleic Acids Research·Dongxue WangXiaodong Cheng
Aug 30, 2017·Proceedings of the National Academy of Sciences of the United States of America·Marian MellénNathaniel Heintz
Jul 15, 2015·The Journal of Biological Chemistry·Hideharu HashimotoXiaodong Cheng
Jan 2, 2019·Experimental Hematology & Oncology·Stephen CaponeGiridharan Ramsingh
Feb 15, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yupeng HeJoseph R Ecker
Dec 20, 2018·Nucleic Acids Research·Jie YangXiaodong Cheng
Oct 23, 2019·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Toby MansellUNKNOWN Barwon Infant Study Investigator Team
May 18, 2018·Biochemical and Biophysical Research Communications·Sreejana RayCharles Vinson
Jul 21, 2021·Life Science Alliance·Husen M UmerClaes Wadelius

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