C/EBP homologous protein-mediated endoplasmic reticulum stress-related renal apoptosis is involved in rats with brain death

Transplantation Proceedings
S CaoS Zhang

Abstract

C/EBP homologous protein (CHOP) is an important marker in endoplasmic reticulum stress (ERS)-associated cell apoptosis. The role of CHOP-induced renal apoptosis remains unclear in rats with brain death (BD). The present study aims to investigate the possible implication of CHOP-mediated, ERS-related BD-induced apoptosis in rats. Forty male Sprague-Dawley rats were divided randomly into 4 experimental groups. We examined activation of ERS and apoptosis-related protein expression using Western blot and immunohistochemical staining. In addition, apoptosis is assessed by terminal deoxynucleotide transferase-mediated dUTP nick-end labeling assay. Kidneys harvested after BD show increased ERS- and apoptosis-related protein markers compared with kidneys of non-BD rats. Salubrinal (Sal) significantly increased levels of p-eIF2a and decreased the activity of CHOP at 6 hours after BD compared with vehicle-treated dimethylsulfoxide. Treating the BD donor with Sal influences renal apoptosis compared with vehicle-treated BD rats. Our results indicate that targeting the CHOP pathway provides a promising therapeutic approach for kidney injury associated with donor BD.

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Citations

Sep 21, 2010·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Flori R SariYoshifusa Aizawa
Jul 17, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·W G LandA Linkermann
Feb 14, 2019·Molecular Biology Reports·Natália Emerim LemosDaisy Crispim
Feb 22, 2018·Physiological Reviews·Maysa SarhanAndreas Linkermann

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