C/EBPalpha directs monocytic commitment of primary myeloid progenitors.

Blood
Dehua WangAlan D Friedman

Abstract

C/EBPalpha is required for generation of granulocyte-monocyte progenitors, but the subsequent role of C/EBPalpha in myeloid lineage commitment remains uncertain. We transduced murine marrow cells with C/EBPalpha-estradiol receptor (ER) or empty vector and subjected these to lineage depletion just prior to culture in estradiol with myeloid cytokines. This protocol limits biases due to lineage-specific effects on developmental kinetics, proliferation, and apoptosis. Also, lowering the dose of estradiol reduced activated C/EBPalpha-ER to near the physiologic range. C/EBPalpha-ER increased Mac1(+)/Gr1(-)/MPO(-)/low monocytes 1.9-fold while reducing Mac1(+)/Gr1(+)/MPO(hi) granulocytes 2.5-fold at 48 hours, even in 0.01 microM estradiol. This pattern was confirmed morphologically and by quantitative polymerase chain reaction (PCR) assay of lineage markers. To directly assess effects on immature progenitors, transduced cells were cultured for 1 day with and then in methylcellulose without estradiol. A 2-fold increase in monocytic compared with granulocytic colonies was observed in IL-3/IL-6/SCF or GM-CSF, but not G-CSF, even in 0.01 microM estradiol. C/EBPalpha-ER induced PU.1 mRNA, and PU.1-ER stimulated monocytic development, sugges...Continue Reading

References

Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·D E ZhangD G Tenen
Sep 2, 2003·Journal of Leukocyte Biology·Tanawan Kummalue, Alan D Friedman
Jan 1, 2004·Molecular and Cellular Biology·Sarah E RossOrmond A MacDougald
May 28, 2004·Cell·Huafeng XieThomas Graf
May 4, 2005·The Journal of Experimental Medicine·Aleksandar DakicStephen L Nutt

❮ Previous
Next ❯

Citations

Dec 24, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Gautam DravidGay M Crooks
Mar 14, 2009·Nature Reviews. Immunology·Rhodri CeredigGeoffrey Brown
Oct 16, 2007·Oncogene·T Pabst, B U Mueller
Jun 1, 2012·Comparative and Functional Genomics·Godfrey Grech, Marieke von Lindern
Dec 19, 2008·PLoS Biology·Andrew B SharabiSi-Yi Chen
Dec 14, 2011·Critical Reviews in Oncogenesis·Ido Paz-Priel, Alan Friedman
Mar 26, 2013·Cellular and Molecular Life Sciences : CMLS·René HuberKorbinian Brand
Dec 16, 2010·The Journal of Clinical Investigation·Daniel KreiselAndrew E Gelman
Dec 25, 2010·Critical Reviews in Clinical Laboratory Sciences·Geoffrey BrownRhodri Ceredig
Oct 13, 2015·British Journal of Haematology·Marije BartelsMarrie Bruin
May 9, 2012·Hormones and Behavior·Rodney M RitzelLouise D McCullough
Jul 30, 2010·Molecular Immunology·Shu-Fen ChiangShiow-Her Chiou
Nov 19, 2008·Cellular Signalling·Louise CarneyAnthony D Whetton
Jan 31, 2008·Cancer Treatment Reviews·Antonio PalumboHermann Einsele
Aug 3, 2007·Blood Cells, Molecules & Diseases·Alan D Friedman
Feb 6, 2007·Journal of Reproductive Immunology·Vivekanand Gupta, Sukh Mahendra Singh
May 17, 2011·British Journal of Haematology·Jennifer M BirdUNKNOWN Haemato-oncology Task Force of British Committee for Standards in Haematology (BCSH) and UK Myeloma Forum
Aug 25, 2015·Mechanisms of Ageing and Development·Cesar SepúlvedaEduardo Fuentes
Jan 27, 2015·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Elizabeth E ZumbrunMitzi Nagarkatti
Sep 21, 2011·Cytokine·A X S SantosF C R Guma
Jul 21, 2007·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Shubham Pant, Edward A Copelan
Jan 13, 2015·Gene Regulation and Systems Biology·Jun-Ichi SatohYoshihiro Kino

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis