C/EBPbeta induces chromatin opening at a cell-type-specific enhancer

Molecular and Cellular Biology
Annette PlachetkaKarl-Heinz Klempnauer

Abstract

We have used the chicken mim-1 gene as a model to study the mechanisms by which transcription factors gain initial access to their target sites in compacted chromatin. The expression of mim-1 is restricted to the myelomonocytic lineage of the hematopoietic system where it is regulated synergistically by the Myb and CCAAT/enhancer binding protein (C/EBP) factors. Myb and C/EBPbeta cooperate at two distinct cis elements of mim-1, the promoter and a cell-type-specific enhancer, both of which are associated with DNase I hypersensitive sites in myelomonocytic cells but not in mim-1-nonexpressing cells. Previous work has shown that ectopic expression of Myb and C/EBPbeta activates the endogenous mim-1 gene in a nonhematopoietic cell type (fibroblasts), where the gene is normally completely silent. Here, we investigated the molecular details of this finding and show that the activation of mim-1 occurs by two independent mechanisms. In the absence of Myb, C/EBPbeta triggers the initial steps of chromatin opening at the mim-1 enhancer without inducing transcription of the gene. mim-1 transcription occurs only in the presence of Myb and is associated with chromatin opening at the promoter. Our work identifies a novel function for C/EBPbe...Continue Reading

References

Mar 1, 1995·Molecular and Cellular Biology·C C Adams, J L Workman
Apr 1, 1996·Molecular and Cellular Biology·S MinkK H Klempnauer
Nov 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D J Steger, J L Workman
Oct 29, 1997·Molecular and Cellular Biology·S MinkK H Klempnauer
Oct 6, 1998·Annual Review of Biochemistry·J L Workman, R E Kingston
Dec 22, 1999·Biochemical and Biophysical Research Communications·M D LaneM S Jiang
Jan 5, 2000·Molecular Cell·E Kowenz-Leutz, A Leutz
Dec 26, 2001·Science's STKE : Signal Transduction Knowledge Environment·H H ChenA D Riggs
Jun 5, 2002·Annual Review of Biochemistry·Peter B Becker, Wolfram Hörz
Feb 8, 2003·The EMBO Journal·Claudia SchwartzKarl-Heinz Klempnauer
Feb 18, 2003·Molecular and Cellular Biology·Joost H A MartensAlt Zantema
Dec 17, 2004·Molecular and Cellular Biology·Olesya ChaykaKarl-Heinz Klempnauer
Jan 25, 2005·Journal of Molecular Biology·Brent Brower-TolandMichelle D Wang
Aug 3, 2006·Genes & Development·Jerry L Workman
Oct 13, 2006·Eukaryotic Cell·Mark ChandyJerry L Workman
Feb 27, 2007·Cell·Tony Kouzarides
Feb 27, 2007·Cell·Bing LiJerry L Workman

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Citations

Oct 28, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Giorgia SantilliAdrian J Thrasher
Aug 20, 2009·The Journal of Biological Chemistry·Nils Wethkamp, Karl-Heinz Klempnauer
Sep 4, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Zhangsheng YangJohn R McCarrey
Mar 1, 2012·Cellular Signalling·René HuberKorbinian Brand
Sep 27, 2020·FEBS Letters·Eugen WerweinKarl-Heinz Klempnauer
Jul 10, 2014·Acta Crystallographica. Section D, Biological Crystallography·Prasenjit BhaumikMaria Miller
Jul 11, 2018·Proceedings of the National Academy of Sciences of the United States of America·Sharon R GrossmanEric S Lander
Nov 4, 2020·International Journal of Molecular Sciences·Maxence CornutThomas Henry
Mar 15, 2020·Cell Stem Cell·Bérengère de LavalMichael H Sieweke
May 1, 2021·International Journal of Molecular Sciences·Ljubomir VitkovMartin Herrmann

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