C/EBPepsilon directs granulocytic-vs-monocytic lineage determination and confers chemotactic function via Hlx.

Experimental Hematology
Stephanie HaleneNancy Berliner

Abstract

Mutations in the CCAAT enhancer binding protein epsilon (C/EBPepsilon) gene have been identified in the cells of patients with neutrophil specific granule deficiency, a rare congenital disorder marked by recurrent bacterial infections. Their neutrophils, in addition to lacking specific granules required for normal respiratory burst activity, also lack normal phagocytosis and chemotaxis. Although the specific granule deficiency phenotype has been replicated in C/EBPepsilon(-/-) (knockout [KO]) mice, the mechanisms by which C/EBPepsilon mutations act to decrease neutrophil function are not entirely clear. In order to determine the role of C/EBPepsilon in neutrophil differentiation and migration, we generated immortalized progenitor cell lines from C/EBPepsilon KO and wild-type mice and performed expression and flow cytometric analysis and functional studies. Expression of lineage-specific cell surface antigens on our in vitro differentiated cell lines revealed persistent expression of monocytic markers on KO granulocytes. We verified this in primary murine peripheral blood and bone marrow cells. In addition, KO bone marrow had an increase in immature myeloid precursors at the common myeloid progenitor and granulocyte/monocyte pro...Continue Reading

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Citations

Jul 5, 2013·PloS One·Carolyn GlassArchibald Perkins
Oct 5, 2013·In Vitro Cellular & Developmental Biology. Animal·Valentina V NenashevaVyacheslav Z Tarantul
Mar 26, 2013·Cellular and Molecular Life Sciences : CMLS·René HuberKorbinian Brand
Feb 28, 2014·Blood·Ashley TaylorStephanie Halene
Jul 9, 2013·Glia·Clara BeutnerHarald Neumann
Jun 28, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Krishnakumar MaluPeter Gaines
Sep 23, 2020·Life Science Alliance·Andreas Fønss Møller, Kedar Nath Natarajan
Aug 6, 2021·Laboratory Medicine·Eman M HabibMuhammad T Abdel Ghafar

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