Cecropin A Modulates Tight Junction-Related Protein Expression and Enhances the Barrier Function of Porcine Intestinal Epithelial Cells by Suppressing the MEK/ERK Pathway

International Journal of Molecular Sciences
Zhenya ZhaiYulong Yin

Abstract

Inflammatory bowel disease (IBD) in humans and animals is associated with bacterial infection and intestinal barrier dysfunction. Cecropin A, an antimicrobial peptide, has antibacterial activity against pathogenic bacteria. However, the effect of cecropin A on intestinal barrier function and its related mechanisms is still unclear. Here, we used porcine jejunum epithelial cells (IPEC-J2) as a model to investigate the effect and mechanism of cecropin A on intestinal barrier function. We found that cecropin A reduced Escherichia coli (E. coli) adherence to IPEC-J2 cells and downregulated mRNA expression of tumor necrosis factor α (TNF-α), interleukin-6 (IL-6), and interleukin-8 (IL-8). Furthermore, cecropin A elevated the transepithelial electrical resistance (TER) value while reducing the paracellular permeability of the IPEC-J2 cell monolayer barrier. Finally, by using Western blotting, immunofluorescence and pathway-specific antagonists, we demonstrated that cecropin A increased ZO-1, claudin-1 and occludin protein expression and regulated membrane distribution and F-actin polymerization by increasing CDX2 expression. We conclude that cecropin A enhances porcine intestinal epithelial cell barrier function by downregulating the...Continue Reading

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Citations

Sep 27, 2019·Journal of Biochemical and Molecular Toxicology·Zhigang ChenHongyu Lei
Oct 2, 2019·International Journal of Molecular Sciences·Ritva Tikkanen, David J Nikolic-Paterson
Jun 17, 2020·Scandinavian Journal of Gastroenterology·Yunling WenYinglei Miao
Oct 31, 2020·Advances in Nutrition·Mark F McCarty, Aaron Lerner
Jul 1, 2020·European Journal of Pharmacology·Maksymilian ZiajaAgnieszka Piastowska-Ciesielska
Jan 3, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Jun-Nan HuWei Li
Jun 23, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Qian LinJun He
Feb 12, 2019·Journal of Agricultural and Food Chemistry·Yue-Bang YinYu-Long Yin

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Methods Mentioned

BETA
PCR
protein assay

Software Mentioned

SAS

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An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.