Cel9D, an atypical 1,4-beta-D-glucan glucohydrolase from Fibrobacter succinogenes: characteristics, catalytic residues, and synergistic interactions with other cellulases.

Journal of Bacteriology
Meng QiCecil W Forsberg

Abstract

The increasing demands of renewable energy have led to the critical emphasis on novel enzymes to enhance cellulose biodegradation for biomass conversion. To identify new cellulases in the ruminal bacterium Fibrobacter succinogenes, a cell extract of cellulose-grown cells was separated by ion-exchange chromatography and cellulases were located by zymogram analysis and identified by peptide mass fingerprinting. An atypical family 9 glycoside hydrolase (GH9), Cel9D, with less than 20% identity to typical GH9 cellulases, was identified. Purified recombinant Cel9D enhanced the production of reducing sugar from acid swollen cellulose (ASC) and Avicel by 1.5- to 4-fold when mixed separately with each of four other glucanases, although it had low activity on these substrates. Cel9D degraded ASC and cellodextrins with a degree of polymerization higher than 2 to glucose with no apparent endoglucanase activity, and its activity was restricted to beta-1-->4-linked glucose residues. It catalyzed the hydrolysis of cellulose by an inverting mode of reaction, releasing glucose from the nonreducing end. Unlike many GH9 cellulases, calcium ions were not required for its function. Cel9D had increased kcat/Km values for cello-oligosaccharides with...Continue Reading

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Citations

Jan 17, 2012·Applied and Environmental Microbiology·Xiaoyun SuIsaac K O Cann
Jul 21, 2009·Journal of Bacteriology·Brian J WatsonSteven W Hutcheson
Jul 9, 2014·Annual Review of Microbiology·Bryan A WhiteHarry J Flint
May 22, 2010·Cellular and Molecular Life Sciences : CMLS·James R Ketudat Cairns, Asim Esen
Oct 25, 2016·Animal Science Journal = Nihon Chikusan Gakkaihō·Takumi ShinkaiAkio Takenaka
Jan 19, 2016·Frontiers in Microbiology·Nurdyana Abdul RahmanPhilip Hugenholtz
Jun 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jaturong KumlaSaisamorn Lumyong
May 26, 2017·Scientific Reports·Chia-Wei WuSteven D Brown
May 30, 2017·Applied Microbiology and Biotechnology·Cheng-Jie DuanJia-Xun Feng
Jun 1, 2018·BMC Evolutionary Biology·Siddhartha Kundu, Rita Sharma
Apr 20, 2021·Applied Biochemistry and Biotechnology·Ravish GodseRam Kulkarni

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