Celastrus paniculatus seed water soluble extracts protect against glutamate toxicity in neuronal cultures from rat forebrain

Journal of Ethnopharmacology
Praful GodkarBhupendra P Doctor

Abstract

Aqueous extracts of Celastrus paniculatus (CP) seed have been reported to improve learning and memory in rats. In addition, these extracts were shown to have antioxidant properties, augmented endogenous antioxidant enzymes, and decreased lipid peroxidation in rat brain. However, water soluble extracts of CP seed (CP-WSE) have not been evaluated for their neuroprotective effects. In the study reported here, we used enriched forebrain primary neuronal cell (FBNC) cultures to study the neuroprotective effects of three CP-WSE extracts (a room temperature, WF; a hot water, HF; and an acid, AF) on glutamate-induced toxicity. FBNC were pre-treated with the CP-WSE and then with glutamate to evaluate the protection afforded against excitatory amino acid-induced toxicity. The criteria for neuroprotection were based on the effects of CP-WSE on a mitochondrial function test following glutamate-induced neurotoxicity. Pre-treatment of neuronal cells with CP-WSE significantly attenuated glutamate-induced neuronal death. To understand the molecular mechanism of action of CP-WSE, we conducted electrophysiological studies using patch-clamp techniques on N-methyl-D-aspartate (NMDA)-activated whole-cell currents in FBNC. WSE significantly and reve...Continue Reading

References

Apr 30, 1992·Biochemical and Biophysical Research Communications·Y AshaniB P Doctor
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C M TangM Morad
Mar 1, 1990·Journal of Neurochemistry·J PatelA Salama
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Oct 29, 1993·Science·J T Coyle, P Puttfarcken
Mar 24, 2000·Free Radical Research·J SastreJ Viña
Aug 24, 2000·Journal of Neuroscience Research·X Q XiaoX C Tang
Apr 24, 2001·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·A RussoA Vanella
Mar 29, 2002·Journal of Applied Toxicology : JAT·R K GordonH S Ved
Jul 18, 2002·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·M H V Kumar, Y K Gupta

❮ Previous
Next ❯

Citations

Jun 10, 2006·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·Wanlop WeecharangsanPongpan Siripong
Jul 4, 2012·Alzheimer's Research & Therapy·Rammohan V RaoDale E Bredesen
Feb 24, 2009·Chemistry & Biodiversity·Xiao-Hui SuHiromasa Kiyota
Jun 4, 2020·Omics : a Journal of Integrative Biology·Sayali Chandrashekhar DeolankarThottethodi Subrahmanya Keshava Prasad
Jul 1, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chintha VenkataramaiahJangampalli Adi Pradeepkiran
Sep 17, 2021·Journal of Complementary & Integrative Medicine·Mohd Aleem

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anti-N-Methyl-D-Aspartate Receptor Encephalitis

Anti-N-Methyl-D-Aspartate (NMDA) receptor encephalitis is a form of of brain inflammation due to antibodies against NMDA receptors. Discover the latest research on anti-NMDA receptor encephalitis here.

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.