Jun 5, 2016

Celecoxib promotes degranulation of CD8(+) T cells in HPV-induced lesions of K14-HPV16 transgenic mice

Life Sciences
Carlos SantosRui M Gil da Costa

Abstract

Human papillomavirus (HPV) is a known biologic carcinogen which is commonly transmitted through sexual intercourse. CD8(+) T cells are known effectors against tumour cells and an important prognostic marker in HPV-induced cancers. COX-2 inhibitors enhance CD8(+) T cell activity against some cancers. In this work, we sought to study the presence and activation of CD8(+) T lymphocytes in lesions from K14-HPV16 transgenic mice and the immunomodulatory effect of celecoxib (CXB) over these cells. Skin samples of CXB-treated and untreated HPV16(-/-) and HPV16(+/-) mice were enzymatically digested and analysed by flow cytometry to assess CD8(+) and CD8(+)CD107a(+) T cell infiltrates. Matched skin samples were classified histologically. HPV16(+/-) mice presented higher CD8(+) T cell infiltration than HPV16(-/-) animals (P<0.001). Older HPV16(+/-) animals showed epidermal dysplasia and increased percentages of CD8(+)CD107a(+) T cells compared with younger animals with hyperplasia (P<0.001), validating this model for testing the effects of celecoxib on CD8(+) T cells. CXB-treated HPV16(+/-) mice showed higher percentages of CD8(+)CD107a(+) T cells compared with untreated HPV16(+/-) animals (P<0.01), but no differences were observed conce...Continue Reading

  • References34
  • Citations6

References

  • References34
  • Citations6

Mentioned in this Paper

Biological Markers
Buffers
Tumor Cells, Uncertain Whether Benign or Malignant
Immune Response
KRT14 protein, human
Flow Cytometry
Immune System
Human Papillomavirus
Epithelial Hyperplasia of Skin
Alb

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