PMID: 7541139Jun 20, 1995Paper

Cell acidification in apoptosis: granulocyte colony-stimulating factor delays programmed cell death in neutrophils by up-regulating the vacuolar H(+)-ATPase

Proceedings of the National Academy of Sciences of the United States of America
R A GottliebB M Babior

Abstract

Neutrophils in tissue culture spontaneously undergo programmed cell death (apoptosis), a process characterized by well-defined morphological alterations affecting the cell nucleus. We found that these morphological changes were preceded by intracellular acidification and that acidification and the apoptotic changes in nuclear morphology were both delayed by granulocyte colony-stimulating factor (G-CSF). Among the agents that defend neutrophils against intracellular acidification is a vacuolar H(+)-ATPase that pumps protons out of the cytosol. When this proton pump was inhibited by bafilomycin A1, G-CSF no longer protected the neutrophils against apoptosis. We conclude that G-CSF delays apoptosis in neutrophils by up-regulating the cells' vacuolar H(+)-ATPase and that intracellular acidification is an early event in the apoptosis program.

References

Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·V DuronioJ W Schrader
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A Nanda, S Grinstein
Jan 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J H WijsmanJ H van Dierendonck
Feb 17, 1995·The Journal of Biological Chemistry·K WongN Hunchuk
Feb 1, 1994·The Journal of Cell Biology·S M Frisch, H Francis
Apr 1, 1994·The Journal of Cell Biology·R C BatesG F Burns
Jan 1, 1993·Archives of Biochemistry and Biophysics·M A Barry, A Eastman
Jun 1, 1993·European Journal of Immunology·B MotykaJ D Reynolds

❮ Previous
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Citations

Oct 15, 1998·The Journal of Pathology·T OhtaS Ohkuma
Aug 24, 2001·International Journal of Cancer. Journal International Du Cancer·T MurakamiK Kohno
Jan 6, 2000·Archives of Biochemistry and Biophysics·K Ollinger
Nov 21, 2007·Pflügers Archiv : European journal of physiology·Ayana HintonMichael Forgac
Nov 8, 2001·European Journal of Pharmacology·O CulićM J Parnham
Jun 1, 1997·Mechanisms of Ageing and Development·T FülöpG Dupuis
Aug 2, 2003·Biochimica Et Biophysica Acta·Hiroto IzumiKimitoshi Kohno
Feb 25, 2000·Brain Research. Molecular Brain Research·H HungE H Lee
Mar 8, 2013·Molecular Pharmaceutics·Xiao-Xiang ZhangXiaoyuan Chen
Nov 2, 2004·Nature Reviews. Cancer·Robert A Gatenby, Robert J Gillies
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Deri MorganThomas E DeCoursey
Jan 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·R A GottliebB M Babior
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·R A Gottlieb, A Dosanjh
Apr 2, 2009·The Journal of Biological Chemistry·Sanjeev KumarYury Ladilov
Sep 22, 2006·Antioxidants & Redox Signaling·Donna P FrazierKeith A Webster
Aug 7, 2013·Journal of Molecular Cell Biology·Hanna AppelqvistKarin Öllinger
Jul 31, 2003·Nucleic Acids Research·Takayuki TorigoeKimitoshi Kohno
May 2, 2002·Anti-cancer Drugs·Takayuki TorigoeKimitoshi Kohno
Dec 23, 1999·Current Opinion in Hematology·P E NewburgerS M Weissman
Jan 1, 1997·Annual Review of Cell and Developmental Biology·T H Stevens, M Forgac
May 15, 1996·The Journal of Clinical Investigation·R A GottliebR L Engler
Aug 3, 1999·The Journal of Clinical Investigation·K A WebsterN H Bishopric
Jul 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N OkahashiT Nishihara
Jan 4, 2003·Photochemistry and Photobiology·Christian Benno OberdannerKristjan Plaetzer
Jul 12, 2002·Stem Cells·H YangE T Papoutsakis
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·H Zieler, J A Dvorak
Sep 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Stephanie A LeeTatiana G Kutateladze
Sep 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lori A KubasiakKeith A Webster
Aug 24, 2010·Cellular and Molecular Life Sciences : CMLS·Anurag Kumar SinghUrsula Seidler
Nov 1, 1996·Stem Cells·D J McConkey, S Orrenius
Jul 1, 1997·Expert Opinion on Investigational Drugs·T D Ingolia, D Platika

❮ Previous
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