Cell adhesion mechanisms: modeling using derivatized beads and sea urchin cell systems

Acta histochemica
R L RoqueS B Oppenheimer

Abstract

Agarose beads derivatized with amino acids, peptides, carbohydrates and lectins were used to systematically determine what types of molecules, isolated from all others, can make adhesive bonds strong enough to hold cell-like beads together. The results indicated that strong adhesion occurred when at least one of the two members of certain bead pairs was derivatized with molecules that were dimers or trimers but not monomers. Also, beads derivatized with phosphorylated amino acids, but not their non-phosphorylated counterparts, adhered to beads derivatized with positively charged peptides. Adhesion was sensitive to ionic strength and pH of the medium. It was proposed that adhesion occurred between the phosphate groups of the phosphoamino acids and amino and guanidinium groups of the peptides. Cooperative bonding can explain the stability of the adhesion observed in this system. Information gained from the bead modeling work was used to design experiments to examine the role of phosphorylated molecules in modulating adhesion in sea urchin systems. Phosphoamino acids inhibited sperm-egg interaction, but not reaggregation of blastula cells. Inhibitors of alkaline phosphatase, however, did inhibit reaggregation. The results suggest ...Continue Reading

References

Mar 1, 1987·The Journal of Cell Biology·T A YednockS D Rosen
Mar 23, 1995·Nature·L ShapiroW A Hendrickson
Aug 1, 1994·Developmental Biology·Y A Abassi, K R Foltz

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Citations

Jan 16, 1998·Acta histochemica·J PhilipS B Oppenheimer
Aug 12, 1999·Acta histochemica·B A SalbillaS B Oppenheimer
May 8, 2002·Acta histochemica·Vanessa M NavarroSteven B Oppenheimer
Oct 23, 2002·Acta histochemica·Maria R KhurrumSteven B Oppenheimer
Apr 2, 2003·Acta histochemica·Lylla NgoSteven B Oppenheimer
Sep 27, 2005·Acta histochemica·Eileen L HeinrichSteven B Oppenheimer
May 19, 2004·Acta histochemica·Maria KhurrumSteven B Oppenheimer
Sep 20, 2011·Chembiochem : a European Journal of Chemical Biology·Morten MeldalGrith Hagel
Sep 2, 2016·Journal of Peptide Science : an Official Publication of the European Peptide Society·Sergey NikitinFrederik Diness

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