Cell Adhesion Molecules and Stem Cell-Niche-Interactions in the Limbal Stem Cell Niche

Stem Cells
Naresh PolisettiUrsula Schlötzer-Schrehardt

Abstract

Interactions between stem cells and their microenvironment are critical for regulation and maintenance of stem cell function. To elucidate the molecular interactions within the human limbal epithelial stem/progenitor cell (LEPC) niche, which is essential for maintaining corneal transparency and vision, we performed a comprehensive expression analysis of cell adhesion molecules (CAMs) using custom-made quantitative real-time polymerase chain reaction (qRT-PCR) arrays and laser capture-microdissected LEPC clusters, comprising LEPCs, melanocytes, mesenchymal cells, and transmigrating immune cells. We show that LEPCs are anchored to their supporting basement membrane by the laminin receptors α3β1 and α6β4 integrin and the dystroglycan complex, while intercellular contacts between LEPCs and melanocytes are mediated by N-, P-, and E-cadherin together with L1-CAM, a member of the immunoglobulin superfamily (Ig)CAMs. In addition to the LEPC-associated heparan sulfate proteoglycans syndecan-2, glypican-3, and glypican-4, the IgCAM members ICAM-1 and VCAM-1 were found to be variably expressed on LEPCs and associated niche cells and to be dynamically regulated in response to chemokines such as interferon-γ to enhance interactions with imm...Continue Reading

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Citations

Apr 12, 2017·Current Opinion in Ophthalmology·Ghasem YazdanpanahAli R Djalilian
Apr 22, 2018·Journal of Cellular and Molecular Medicine·Ping GuoYingting Zhu
Jan 8, 2019·Cornea·Sophie X DengUNKNOWN and The International Limbal Stem Cell Deficiency Working Group
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Jul 8, 2020·Scientific Reports·Naresh PolisettiUrsula Schlötzer-Schrehardt
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Feb 6, 2021·Scientific Reports·Naresh PolisettiThomas Reinhard
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Aug 27, 2021·Bioengineering·Mohamed Abdul-AlFarshid Sefat
Aug 24, 2021·The Ocular Surface·Naresh PolisettiUrsula Schlötzer-Schrehardt

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