PMID: 6970200Dec 1, 1980Paper

Cell adhesion molecules: detection with univalent second antibody

The Journal of Cell Biology
W R Springer, S H Barondes

Abstract

Identification of cell surface molecules that play a role in cell-cell adhesion (here called cell adhesion molecules) has been achieved by demonstrating the inhibitory effect of univalent antibodies that bind these molecules in an in vitro assay of cell-cell adhesion. A more convenient reagent, intact (divalent) antibody, has been avoided because it might agglutinate the cells rather than blocking cell-cell adhesion. In this report, we show that intact rabbit immunoglobulin directed against certain cell surface molecules of Dictyostelium discoideum blocks cell-cell adhesion when the in vitro assay is performed in the presence of univalent goat anti-rabbit antibody. Under appropriate experimental conditions, the univalent second antibody blocks agglutination induced by the rabbit antibody without significantly interfering with its effect on cell-cell adhesion. This method promises to be useful for screening monoclonal antibodies raised against potential cell adhesion molecules because: (a) it allows for the screening of large numbers of antibody samples without preparation of univalent fragments; and (b) it requires much less antibody because of the greater affinity of divalent antibodies for antigens.

References

Apr 5, 1978·Journal of Molecular Biology·S Bozzaro, G Gerisch
Sep 1, 1978·The Journal of Cell Biology·W R Springer, S H Barondes
Jan 1, 1966·International Archives of Allergy and Applied Immunology·P J McConahey, F J Dixon

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Citations

Aug 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C H Siu
May 1, 1985·Developmental Biology·W R Springer, S H Barondes
May 1, 1987·Developmental Biology·D A KnechtW F Loomis
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W R Springer, S H Barondes
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E A Berger, D R Armant
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C H SiuT Y Lam
Aug 1, 1983·Experimental Cell Research·W F LoomisT Jongens
Sep 1, 1985·Cell·H J GabiusS H Barondes
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·B A MurrayW F Loomis
Mar 1, 1984·Molecular and Cellular Biology·B A MurrayW F Loomis

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