PMID: 16629399Apr 25, 2006Paper

Cell adhesion profiling using extracellular matrix protein microarrays

BioTechniques
Cornelia KuschelBrigitte Angres

Abstract

We have developed a microarray-based system for cell adhesion profiling of large panels of cell-adhesive proteins to increase the throughput of in vitro cell adhesion assays, which are currently primarily performed in multiwell plates. Miniaturizing cell adhesion assays to an array format required the development of protocols for the reproducible microspotting of extracellular matrix (ECM) protein solutions and for the handling of cell suspensions during the assay. We generated ECM protein microarrays with high reproducibility in microspot protein content using nitrocellulose-coated glass microslides, combined with piezoelectric microspotting of protein solutions. Protocols were developed that allowed us to use 5000 cells or fewer on an array of 4 x 4 mm consisting of 64 microspots. Using this microarray system, we identified differences of adhesive properties of three cell lines to 14 different ECM proteins. Furthermore, the sensitivity and accuracy of the assays were increased using microarrays with ranges of ECM protein amounts. This microarray system will be particularly useful for extensive comparative cell adhesion profiling studies when only low amounts of adhesive substrate and cells, such as stem cells or cells from bi...Continue Reading

References

Sep 29, 1995·The Journal of Biological Chemistry·L M SchnappR Pytela
Nov 17, 1999·Proceedings of the Society for Experimental Biology and Medicine·G J Mizejewski
May 10, 2000·The Journal of Biological Chemistry·E F PlowJ W Smith
Sep 27, 2001·Cell and Tissue Research·A van der Flier, A Sonnenberg
Dec 12, 2001·Circulation Research·B P Eliceiri
Apr 11, 2002·Nature Cell Biology·Martin A Schwartz, Mark H Ginsberg
Jun 18, 2003·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Brigitte Lelongt, Pierre Ronco
Nov 26, 2003·Glycobiology·Leonardo NimrichterRonald L Schnaar
Dec 3, 2003·The Journal of Pathology·Erik H J Danen, Arnoud Sonnenberg
Mar 25, 2004·Journal of Cellular Physiology·Francesco RossoAlfonso Barbarisi
Jun 15, 2004·Nature Biotechnology·Daniel G AndersonRobert Langer
Jun 23, 2004·Biomaterials·Yoshihiro ItoTohru Shibuya
Mar 23, 2005·Nature Methods·Christopher J FlaimSangeeta N Bhatia

❮ Previous
Next ❯

Citations

Nov 22, 2008·Analytical and Bioanalytical Chemistry·Michael HartmannJohan Roeraade
Mar 21, 2009·Journal of Materials Science. Materials in Medicine·Santiago A Rodríguez-SeguíJosep Samitier
Dec 4, 2013·Advanced Drug Delivery Reviews·Maria HåkansonMirren Charnley
May 25, 2007·Nature Reviews. Molecular Cell Biology·Shin-Ichi NishikawaTakumi Era
Feb 15, 2008·Stem Cells and Development·Christopher J FlaimSangeeta N Bhatia
Feb 19, 2010·Tissue Engineering. Part B, Reviews·Lilia Araida Hidalgo-Bastida, Sarah H Cartmell
Sep 26, 2008·BMC Genomics·Angelika OehmigHansjürgen Volkmer
Feb 1, 2013·Journal of Translational Medicine·Karin BenzWilhelm K Aicher
Dec 3, 2010·Journal of Cell Science·Christian FrantzValerie M Weaver
Feb 9, 2007·Pharmacogenomics·Catherine Putonti
May 15, 2013·Expert Opinion on Drug Delivery·Saradha ChandrasekharVasant V Ranade
Sep 8, 2016·Microarrays·Rebecca JonczykFrank Stahl
Mar 28, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Patricia S HeggerHeike Boehm
Oct 4, 2013·Journal of Molecular Recognition : JMR·Lu DaoClemens M Franz
Mar 31, 2010·Cellular and Molecular Life Sciences : CMLS·Yvonne BaumerBurkhard Schlosshauer
Feb 28, 2007·Chemical Society Reviews·Juan J Díaz-MochónMark Bradley
Mar 28, 2012·Journal of Thrombosis and Haemostasis : JTH·Y BaumerM Gawaz
Jun 1, 2011·Journal of Biomedical Materials Research. Part a·Santiago A Rodríguez-SeguíJosep Samitier
Nov 16, 2010·Lab on a Chip·Koji HattoriToshiyuki Kanamori
May 30, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Paul J SmithRachel J Errington
Oct 14, 2017·Scientific Reports·Trisha M WesterhofEdward L Nelson
Jan 25, 2011·Acta Biochimica Et Biophysica Sinica·Lina YangShengce Tao
Mar 21, 2020·Cancer Research·Casimiro GerarduzziElliot Drobetsky
May 18, 2021·Nature Reviews. Materials·Benjamin J BielajewKyriacos A Athanasiou
Jun 5, 2021·Advanced Drug Delivery Reviews·Leon Jia Wei TangEliza Li Shan Fong

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.