Cell adhesion receptors for native and denatured type I collagens and fibronectin in rabbit arterial smooth muscle cells in culture
Abstract
Cell adhesion molecules serve as specific cell surface receptors for extracellular matrices and contribute to the attachment, spreading, proliferation, and differentiation of vascular cells. We examined the cell adhesion receptors and binding sites on native type I collagen, heat-denatured type I collagen, and fibronectin in rabbit arterial smooth muscle cells (SMC) in culture. On fibronectin, anti-alpha 3 beta 1 and anti-alpha 5 beta 1 integrin antibodies and the synthetic peptide GRGDSP (Gly-Arg-Gly-Asp-Ser-Pro) significantly inhibited the attachment and spreading of rabbit SMC after 1 and 24 h of culture, while anti-alpha 1 beta 1 inhibited attachment and spreading only after 1 h. In contrast, the attachment and spreading of the cells on native type I collagen were mediated by alpha 1 beta 1 integrin and the cell-binding sequence which did not contain RGD (Arg-Gly-Asp) and DGEA (Asp-Gly-Glu-Ala) after both 1 and 24 h. On heat-denatured type I collagen, alpha 2 beta 1 integrin mediated the cell attachment and spreading after 1 and 24 h and DGEA served as a recognition site for the alpha 2 beta 1 integrin. alpha 1 beta 1 and alpha 3 beta 1 integrins affected only the initial adherence (1 h after plating) of the cells to denatu...Continue Reading
Citations
Urokinase receptor-dependent upregulation of smooth muscle cell adhesion to vitronectin by urokinase
Angiotensin II and PDGF-BB stimulate beta(1)-integrin-mediated adhesion and spreading in human VSMCs
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Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.