Cell adhesion receptors for native and denatured type I collagens and fibronectin in rabbit arterial smooth muscle cells in culture

Experimental Cell Research
K Yamamoto, M Yamamoto

Abstract

Cell adhesion molecules serve as specific cell surface receptors for extracellular matrices and contribute to the attachment, spreading, proliferation, and differentiation of vascular cells. We examined the cell adhesion receptors and binding sites on native type I collagen, heat-denatured type I collagen, and fibronectin in rabbit arterial smooth muscle cells (SMC) in culture. On fibronectin, anti-alpha 3 beta 1 and anti-alpha 5 beta 1 integrin antibodies and the synthetic peptide GRGDSP (Gly-Arg-Gly-Asp-Ser-Pro) significantly inhibited the attachment and spreading of rabbit SMC after 1 and 24 h of culture, while anti-alpha 1 beta 1 inhibited attachment and spreading only after 1 h. In contrast, the attachment and spreading of the cells on native type I collagen were mediated by alpha 1 beta 1 integrin and the cell-binding sequence which did not contain RGD (Arg-Gly-Asp) and DGEA (Asp-Gly-Glu-Ala) after both 1 and 24 h. On heat-denatured type I collagen, alpha 2 beta 1 integrin mediated the cell attachment and spreading after 1 and 24 h and DGEA served as a recognition site for the alpha 2 beta 1 integrin. alpha 1 beta 1 and alpha 3 beta 1 integrins affected only the initial adherence (1 h after plating) of the cells to denatu...Continue Reading

Citations

Jan 1, 1996·The Journal of Cell Biology·J Xu, R A Clark
Aug 8, 2008·American Journal of Physiology. Cell Physiology·Lavanya BalasubramanianChun-Min Lo
Oct 27, 2004·American Journal of Respiratory and Critical Care Medicine·Trang T-B NguyenStuart J Hirst
Dec 4, 2012·Stem Cell Research & Therapy·Min Zhang, Bing Huang
Apr 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Véronique Proux-GillardeauxThierry Galli
Jul 29, 1998·The Journal of Cell Biology·A PozziH A Gardner
Aug 13, 2015·Journal of Biomedical Materials Research. Part a·Marta MadaghieleAlessandro Sannino
Sep 16, 2006·The Journal of Investigative Dermatology·Manon C ZweersBeate Eckes
Mar 5, 2002·The Journal of Investigative Dermatology·Greg SzulgitHumphrey Gardner
Sep 13, 2006·British Journal of Pharmacology·J V BonacciA G Stewart
Mar 7, 2006·Biochemical and Biophysical Research Communications·Susan M SmithMatt K Lee
Aug 30, 2002·Biochemical and Biophysical Research Communications·Florian BlaschkeKristof Graf
Nov 8, 2005·European Journal of Cell Biology·Maria Antonietta BelisarioElisa Gionti
Sep 2, 1999·Experimental Cell Research·B S KimD J Mooney
Feb 13, 2001·Journal of Cellular Physiology·A J MillisC L Moulson
Jul 6, 2000·Journal of Biomedical Materials Research·G JohnsonJ G Steele
May 11, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·B SteffensenH Larjava
Oct 25, 2003·Circulation Research·Harry MaJoan W Berman
Dec 16, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·A W ChangS S Okada
Sep 5, 2002·Circulation·David SimperNoel M Caplice
May 31, 2012·Annals of Plastic Surgery·Alan D Widgerow
Dec 20, 2018·American Journal of Physiology. Cell Physiology·Michael SaemischHeiko Methe
Aug 23, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Jean-Hugues ParmentierKafait U Malik
Nov 16, 2013·Lasers in Medical Science·Michal HegerMartin J C van Gemert
May 2, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Patricia E McCallinhartAaron J Trask
Oct 27, 2020·Aging Medicine·Mingyi WangKimberly R McGraw
Aug 31, 2000·Biomaterials·J Nikolovski, D J Mooney
Mar 21, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·T J McCannF McDonald

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.