Cell-adhesive RGD peptide-displaying M13 bacteriophage/PLGA nanofiber matrices for growth of fibroblasts

Biomaterials Research
Yong Cheol ShinDong-Wook Han

Abstract

M13 bacteriophages can be readily fabricated as nanofibers due to non-toxic bacterial virus with a nanofiber-like shape. In the present study, we prepared hybrid nanofiber matrices composed of poly(lactic-co-glycolic acid, PLGA) and M13 bacteriophages which were genetically modified to display the RGD peptide on their surface (RGD-M13 phage). The surface morphology and chemical composition of hybrid nanofiber matrices were characterized by scanning electron microscopy (SEM) and Raman spectroscopy, respectively. Immunofluorescence staining was conducted to investigate the existence of M13 bacteriophages in RGD-M13 phage/PLGA hybrid nanofibers. In addition, the attachment and proliferation of three different types of fibroblasts on RGD-M13 phage/PLGA nanofiber matrices were evaluated to explore how fibroblasts interact with these matrices. SEM images showed that RGD-M13 phage/PLGA hybrid matrices had the non-woven porous structure, quite similar to that of natural extracellular matrices, having an average fiber diameter of about 190 nm. Immunofluorescence images and Raman spectra revealed that RGD-M13 phages were homogeneously distributed in entire matrices. Moreover, the attachment and proliferation of fibroblasts cultured on RG...Continue Reading

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Citations

May 7, 2016·Chemical Society Reviews·Amy M Wen, Nicole F Steinmetz
Aug 28, 2017·Tissue Engineering and Regenerative Medicine·Jun Hee LeeSang-Mo Kwon
Jul 18, 2019·Nanomaterials·Iruthayapandi Selestin RajaDong-Wook Han
Nov 20, 2018·Advanced Drug Delivery Reviews·Binrui CaoChuanbin Mao
Aug 21, 2019·Biomacromolecules·Seon Yeong ChaeSo Young Yoo

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Methods Mentioned

BETA
PCR
genetic modification
ELISA

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