Cell-associated ovalbumin is cross-presented much more efficiently than soluble ovalbumin in vivo

The Journal of Immunology : Official Journal of the American Association of Immunologists
M LiW R Heath

Abstract

To better understand the antigenic requirements for cross-presentation, we compared the in vivo efficiency of presentation of cell-associated vs soluble OVA with the OT-I (CD8) and OT-II (CD4) TCR transgenic lines. Cross-presentation of cell-associated OVA was very efficient, requiring as little as 21 ng of OVA to activate OT-II cells and 100-fold less to activate OT-I cells. In contrast, soluble OVA was presented inefficiently, requiring at least 10,000 ng OVA for activation of either T cell subset. Thus, cell-associated OVA was presented 500-fold more efficiently than soluble OVA to CD4 T cells and 50,000-fold more efficiently to CD8 T cells. These data, which represent the first quantitative in vivo analysis of cross-presentation, show that cell-associated OVA is very efficiently presented via the class I pathway.

References

Feb 1, 1990·The Journal of Experimental Medicine·F R Carbone, M J Bevan
May 2, 1994·Journal of Immunological Methods·A B Lyons, C R Parish
Jan 14, 1994·Cell·K A HogquistF R Carbone
Jun 1, 1996·The Journal of Experimental Medicine·S OehenS M Hedrick
Oct 1, 1996·Immunity·M JondalJ Reimann
Sep 1, 1996·The Journal of Experimental Medicine·C KurtsH Kosaka
Jul 7, 1997·The Journal of Experimental Medicine·S R BennettW R Heath
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·J S BoyleA M Lew
Jun 13, 1998·The Journal of Experimental Medicine·W R HeathF R Carbone
Aug 26, 1998·Immunology Today·F R CarboneW R Heath
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·C KurtsW R Heath
Dec 20, 2000·The Journal of Experimental Medicine·J M den HaanM J Bevan

❮ Previous
Next ❯

Citations

Feb 28, 2002·Annual Review of Immunology·Pierre GuermonprezSebastian Amigorena
Jul 6, 2004·Immunological Reviews·William R HeathJose A Villadangos
Nov 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hong Zheng, Zihai Li
Dec 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Scott B LovitchEmil R Unanue
Dec 16, 2006·International Reviews of Immunology·Robert J Binder
Jun 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Melanie Werner-KleinThomas Brocker
Oct 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dorothee BourgesAndrew M Lew
Jan 30, 2008·Immunology and Cell Biology·Ian A Parish, William R Heath
Sep 22, 2010·Oncogene·H J SteerB W S Robinson
Dec 5, 2012·Autoimmune Diseases·Robert J BinderSudesh Pawaria
Feb 16, 2013·Journal of the American Society of Nephrology : JASN·Catherine GottschalkChristian Kurts
Jul 23, 2014·Oncoimmunology·Yu Jerry Zhou, Robert Julian Binder
Aug 30, 2016·JCI Insight·Anna Cline-SmithRajeev Aurora
Aug 12, 2003·Nature Medicine·Markus FeuererVolker Schirrmacher
Oct 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Catherine RushPaul Garside
Jul 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mojca SkoberneGernot Geginat
Feb 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Valérie S ZimmermannAngelo A Manfredi
Feb 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Lianjun Shen, Kenneth L Rock
Apr 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Adam P UldrichDale I Godfrey
Mar 22, 2002·Nature Reviews. Immunology·W R Heath, F R Carbone
Apr 9, 2008·Expert Review of Vaccines·Robert J Binder
Feb 17, 2006·Cancer Biotherapy & Radiopharmaceuticals·Xueshu ZhangJim Xiang
Mar 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sammy BedouiElodie Segura
Nov 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fiona KupresaninGabrielle T Belz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.