Cell-attached single-channel recordings in intact prefrontal cortex pyramidal neurons reveal compartmentalized D1/D5 receptor modulation of the persistent sodium current

Frontiers in Neural Circuits
N A Gorelova, J K Seamans

Abstract

The persistent Na(+) current (I(Nap)) is believed to be an important target of dopamine modulation in prefrontal cortex (PFC) neurons. While past studies have tested the effects of dopamine on I(Nap), the results have been contradictory largely because of difficulties in measuring I(Nap) using somatic whole-cell recordings. To circumvent these confounds we used the cell-attached patch-clamp technique to record single Na(+) channels from the soma, proximal dendrite (PD) or proximal axon (PA) of intact prefrontal layer V pyramidal neurons. Under baseline conditions, numerous well resolved Na(+) channel openings were recorded that exhibited an extrapolated reversal potential of 73 mV, a slope conductance of 14-19 pS and were blocked by tetrodotoxin (TTX). While similar in most respects, the propensity to exhibit prolonged bursts lasting >40 ms was many fold greater in the axon than the soma or dendrite. Bath application of the D1/D5 receptor agonist SKF81297 shifted the ensemble current activation curve leftward and increased the number of late events recorded from the PD but not the soma or PA. However, the greatest effect was on prolonged bursting where the D1/D5 receptor agonist increased their occurrence 3 fold in the PD and n...Continue Reading

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Citations

Oct 27, 2015·Frontiers in Neural Circuits·M Victoria PuigGuillermo Gonzalez-Burgos
Mar 14, 2007·International Review of Neurobiology·Yukiori Goto, Anthony A Grace
Jul 16, 2013·Journal of Physiology, Paris·Hidehiko Takahashi
Sep 23, 2016·Biophysical Journal·Elizabeth J AkinMichael M Tamkun
May 6, 2014·Schizophrenia Research·Elmar FrankBerthold Langguth
Sep 16, 2017·Developmental Neurobiology·Maciej GawlakPaweł Szulczyk
Feb 15, 2018·Frontiers in Neuroanatomy·Gabriele Radnikow, Dirk Feldmeyer

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Software Mentioned

pClamp
Clampfit
Statistica

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