Cell-based assays: fuelling drug discovery

Analytical and Bioanalytical Chemistry
Elisa MicheliniAldo Roda

Abstract

It has been estimated that over a billion dollars in resources can be consumed to obtain clinical approval, and only a few new chemical entities are approved by the US Food and Drug Administration (FDA) each year. Therefore it is of utmost importance to obtain the maximum amount of information about biological activity, toxicological profile, biochemical mechanisms, and off-target interactions of drug-candidate leads in the earliest stages of drug discovery. Cell-based assays, because of their peculiar advantages of predictability, possibility of automation, multiplexing, and miniaturization, seem the most appealing tool for the high demands of the early stages of the drug-discovery process. Nevertheless, cellular screening, relying on different strategies ranging from reporter gene technology to protein fragment complementation assays, still presents a variety of challenges. This review focuses on main advantages and limitations of different cell-based approaches, and future directions and trends in this fascinating field.

References

Apr 5, 2003·Biotechnology Progress·Tai Hyun Park, Michael L Shuler
May 26, 2004·Trends in Biotechnology·Aldo RodaMassimo Guardigli
Mar 1, 2005·Analytical Chemistry·Ramasamy Paulmurugan, Sanjiv S Gambhir
Apr 30, 2005·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Heng-Keang LimKelvin Chan
Aug 30, 2005·Analytical Biochemistry·Bruce R BranchiniAldo Roda
Jun 9, 2006·Journal of Biomolecular Screening·Philip E BrandishWei Zheng
Jun 29, 2006·Lab on a Chip·Cheuk-Wing LiMengsu Yang
Oct 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ramasamy Paulmurugan, Sanjiv S Gambhir
Nov 14, 2006·Nature Methods·Ingrid Remy, Stephen W Michnick
Nov 23, 2006·Assay and Drug Development Technologies·Edward VerdonkVivian F Liu
Jan 30, 2007·Journal of Biomolecular Screening·Zhengrong ZhuAngela Cacace
Mar 16, 2007·Assay and Drug Development Technologies·Frank Fan, Keith V Wood
Aug 9, 2007·Nature Reviews. Molecular Cell Biology·Francesco PampaloniErnst H K Stelzer
Aug 21, 2007·Journal of Biotechnology·Dayu LiuBingcheng Lin
Aug 22, 2007·The Analyst·Fareid Asphahani, Miqin Zhang
Aug 28, 2007·Angewandte Chemie·Akira KannoTakeaki Ozawa
Dec 7, 2007·Chemistry, an Asian Journal·Asami Kaihara, Yoshio Umezawa
Jan 4, 2008·Cancer Research·Carmel T ChanSanjiv Sam Gambhir
Jan 12, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jun NakanishiMizuo Maeda
Jan 29, 2008·Biosensors & Bioelectronics·Fareid AsphahaniMiqin Zhang
Jan 30, 2008·Biotechnology Journal·Johan BacartCyril Couturier
Mar 29, 2008·Expert Opinion on Drug Metabolism & Toxicology·James J CaliJohn Shultz
Apr 17, 2008·Biotechnology Journal·Biao XiYama A Abassi
Apr 19, 2008·Analytica Chimica Acta·Tatsuro EndoEiichi Tamiya
Jun 7, 2008·Analytical Chemistry·Kathryn E LukerGary D Luker
Jun 10, 2008·Combinatorial Chemistry & High Throughput Screening·Ye FangRonald Verklereen
Jul 1, 2008·Nature Methods·Paulina CarribaRafael Franco

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Citations

Sep 17, 2011·Analytical and Bioanalytical Chemistry·Elisa Michelini, Aldo Roda
Jun 12, 2013·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Susanna NybondPäivi Tammela
May 26, 2011·Journal of Laboratory Automation·Siegfried F GrafHelmut F Knapp
Jul 13, 2011·Journal of Agricultural and Food Chemistry·Kiyoko NagahamaMasahito Suiko
Sep 29, 2012·Nature Methods·Ken C-C Cheng, James Inglese
Apr 14, 2012·The Analyst·Fareid AsphahaniMiqin Zhang
Feb 23, 2012·SAR and QSAR in Environmental Research·G Panagiotou, O Taboureau
May 18, 2013·Assay and Drug Development Technologies·Kerstin HeiseFrank Gaunitz
Nov 20, 2012·Molekuliarnaia biologiia·S P ChumakovP M Chumakov
Feb 12, 2014·Molecular Biotechnology·Viktoryia SidarovichAlessandro Quattrone
Jul 14, 2012·Expert Opinion on Drug Discovery·Daniel V LaBarberaByong Hoon Yoo
Jul 16, 2013·Drug Discovery Today·Wei ZhengJohn C McKew
Jan 23, 2013·Trends in Biotechnology·Eelco FennemaJan de Boer
Apr 1, 2014·Genes, Brain, and Behavior·R G KrugK J Clark
Sep 8, 2012·Current Opinion in Pharmacology·Madhura Kelkar, Abhijit De
Aug 28, 2012·Trends in Biotechnology·Sofia A M MartinsD Miguel F Prazeres
May 23, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Geeta MehtaShuichi Takayama
Dec 29, 2015·Analytica Chimica Acta·Guansheng DuJaap M J den Toonder
Apr 30, 2015·Scientific Reports·Alejandro Rojas-FernandezGopal P Sapkota
Jun 15, 2011·Birth Defects Research. Part C, Embryo Today : Reviews·Xiaoqian HuangZhiyuan Gong
Nov 26, 2010·British Journal of Pharmacology·J P HughesK L Philpott
Mar 15, 2016·Expert Opinion on Drug Discovery·Ivan Fraietta, Fabio Gasparri
Jul 3, 2015·Biotechnology Advances·Mario RothbauerPeter Ertl
Jan 28, 2014·Advanced Drug Delivery Reviews·Michiya MatsusakiMitsuru Akashi
May 28, 2014·Journal of Biotechnology·May LyShyamsundar Subramanian
May 25, 2016·Free Radical Research·Luís Cláudio Nascimento da SilvaRegina Célia Bressan Queiroz de Figueiredo
Jan 14, 2017·Photochemistry and Photobiology·Luca CeveniniAldo Roda
Jun 3, 2017·International Journal of Molecular Sciences·Logan GanzenMingzhi Zhang
Mar 1, 2017·Journal of Bacteriology·Brittany L NairnPhillip E Klebba
Nov 27, 2014·Journal of Laboratory Automation·David J GuckenbergerDavid J Beebe
Nov 6, 2018·Expert Opinion on Drug Discovery·Aishwarya SatpathyIbrahim T Ozbolat
Dec 21, 2010·Cellular and Molecular Bioengineering·Nazgul Tuleuova, Alexander Revzin
Apr 27, 2019·Antibiotics·Bernardo Ribeiro da CunhaCecília R C Calado

❮ Previous
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