Cell behavior of the highly sticky bacterium Acinetobacter sp. Tol 5 during adhesion in laminar flows

Scientific Reports
Yoshihide FuruichiKatsutoshi Hori

Abstract

It is important to characterize how medically, industrially, or environmentally important bacteria adhere to surfaces in liquid flows in order to control their cell adhesion and subsequent biofilm formation. Acinetobacter sp. Tol 5 is a remarkably sticky bacterium that autoagglutinates through the adhesive nanofiber protein AtaA, which is applicable to cell immobilization in bioprocesses. In this study, the adhesion and behavior of Tol 5 cells in laminar flows were investigated using flow cell systems. Tol 5 cells autoagglutinated through AtaA and formed cell clumps during flowing. The cell clumps rather than single cells went downward due to gravity and adhered to the bottom surface. Under appropriate shear stress, a twin vortex was caused by a separated flow generated at the rear of the pre-immobilized cell clumps and carried the small cell clumps to this location, resulting in their stacking there. The rearward immobilized cell clumps developed into a large, stable aggregate with a streamlined shape, independent of cell growth. Cell clumps hardly ever developed under weak shear stress that could not generate a twin vortex and were broken up under excessively strong shear stress. These cell behaviors including the importance ...Continue Reading

References

Jul 27, 2002·Annual Review of Microbiology·P StoodleyJ W Costerton
Mar 26, 2004·Nature Reviews. Microbiology·Luanne Hall-StoodleyPaul Stoodley
Sep 25, 2004·Molecular Microbiology·Wendy E ThomasViola Vogel
Oct 30, 2004·Nucleic Acids Research·David MetzgarValérie de Crécy-Lagard
Feb 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Tanja Heise, Petra Dersch
Aug 21, 2007·Journal of Bacteriology·Mary Jo KirisitsMatthew R Parsek
Oct 22, 2008·Journal of Bioscience and Bioengineering·Hisami WatanabeKatsutoshi Hori
Jan 20, 2011·Biophysical Journal·Sigolene LecuyerHoward A Stone
Feb 11, 2012·Journal of Bioscience and Bioengineering·Masahito IshikawaKatsutoshi Hori
Aug 11, 2012·Biofouling·Shanika A CruszPaul Williams
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Apr 7, 2015·Applied Microbiology and Biotechnology·Katsutoshi HoriHajime Nakatani
Mar 8, 2016·Nature Communications·Maximilian M SauerRudi Glockshuber
Aug 30, 2016·Cell Reports·Rhodaba EbadyTara J Moriarty
Aug 31, 2016·Nature Microbiology·Minyoung Kevin KimHoward A Stone
Apr 12, 2017·Proceedings of the National Academy of Sciences of the United States of America·Alexandra F NiddamTara J Moriarty

❮ Previous
Next ❯

Citations

Aug 31, 2020·MicrobiologyOpen·Hawzeen S KhalilJack C Leo
Jun 11, 2021·Biochemical Society Transactions·El-Shama Q A Nwoko, Iruka N Okeke
Aug 21, 2021·Journal of Colloid and Interface Science·Satoshi IshiiKatsutoshi Hori

❮ Previous
Next ❯

Methods Mentioned

BETA
enzymatic treatments
PCR

Software Mentioned

ImageJ

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.