PMID: 9436362Jan 22, 1998Paper

Cell-biological changes on the disordered tissues

Nihon hōigaku zasshi = The Japanese journal of legal medicine
T Tsuji

Abstract

In this study, we investigated cell-biologically the cell death generating by injuries, on the basis of two mechanisms of the cell death, these are necrosis and apoptosis. To heat the liver of rats, a heat device having a PID built-in electron regulator system was newly developed by us. The liver of rats laparotomized under anesthesia was heated at 37 degrees C, 50 degrees C and 65 degrees C for 5 min with a heated needle connected to the device, and thereafter the rats were survived in the given time. Appearance of the cell death in the tissue injured by heat was analyzed, in progress of the survival time, by means of staining of the tissue with hematoxylin-eosin (HE) and TUNEL method and agarosegel electrophoresis of DNA extracted from the tissue injured. Consequently, apoptosis appeared, in all heat temperatures, with bimodal pattern showing peak at about 2 and 10 hours after heat. Apoptosis in the first peak dottedly appeared, in heat temperature at 37 degrees C, in the whole region affected by heat. When the liver was heated at 50 degrees C and 65 degrees C, it first appeared on outer edge in the region affected by heat, and then made progress to the center of the region, condensation of chromatin was, in HE staining, rema...Continue Reading

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis