Cell-cell contacts protect against t-BuOOH-induced cellular damage and ferroptosis in vitro

Archives of Toxicology
Christine WenzCornelia Dietrich

Abstract

Ferroptosis is a recently discovered pathway of regulated necrosis dependent on iron and lipid peroxidation. It has gained broad attention since it is a promising approach to overcome resistance to apoptosis in cancer chemotherapy. We have recently identified tertiary-butyl hydroperoxide (t-BuOOH) as a novel inducer of ferroptosis. t-BuOOH is a widely used compound to induce oxidative stress in vitro. t-BuOOH induces lipid peroxidation and consequently ferroptosis in murine and human cell lines. t-BuOOH additionally results in a loss of mitochondrial membrane potential, formation of DNA double-strand breaks, and replication block. Here, we specifically address the question whether cell-cell contacts regulate t-BuOOH-induced ferroptosis and cellular damage. To this end, murine NIH3T3 or human HaCaT cells were seeded to confluence, but below their saturation density to allow the establishment of cell-cell contacts without inducing quiescence. Cells were then treated with t-BuOOH (50 or 200 µM, respectively). We revealed that cell-cell contacts reduce basal and t-BuOOH-triggered lipid peroxidation and consequently block ferroptosis. Similar results were obtained with the specific ferroptosis inducer erastin. Cell-cell contacts fur...Continue Reading

References

Mar 1, 1988·The Journal of Cell Biology·P BoukampN E Fusenig
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Mar 18, 1967·Nature·H Eagle, E M Levine
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G BellomoS Orrenius
Aug 3, 2001·Nature Reviews. Molecular Cell Biology·M Leist, M Jäättelä
Aug 30, 2001·Proceedings of the National Academy of Sciences of the United States of America·T BrabletzT Kirchner
Mar 14, 2002·Biochemical and Biophysical Research Communications·Cornelia DietrichFranz Oesch
Sep 5, 2002·Journal of Cellular Physiology·Raymond C RancourtMichael A O'Reilly
Jul 20, 2005·Oncogene·Dagmar FaustCornelia Dietrich
Jan 30, 2007·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Wai Hoe NgHeng Phon Too
Feb 13, 2007·Cancer Cell·Ignacio DoladoAngel R Nebreda
Jan 15, 2008·Chemical Research in Toxicology·Yinsheng Wang
Jun 24, 2009·Nature Reviews. Molecular Cell Biology·Peter Friedl, Darren Gilmour
Jul 7, 2009·Biochimica Et Biophysica Acta·John J LemastersAnna-Liisa Nieminen
Jan 12, 2010·Mutation Research·Olga A SedelnikovaWilliam M Bonner
Jun 22, 2010·Journal of Cellular Biochemistry·Monika KüppersCornelia Dietrich
Nov 10, 2010·PloS One·Laura J Lewis-TuffinPanos Z Anastasiadis
Feb 12, 2011·The Biochemical Journal·Simon V Avery
Aug 9, 2011·Molecular and Cellular Biochemistry·Marian GaballahDorothy Hutter-Lobo
Sep 7, 2011·International Journal of Oncology·Takahiro YamauchiHisataka Moriwaki
Nov 15, 2011·Cell Death and Differentiation·N VanlangenakkerP Vandenabeele
Dec 6, 2011·The Journal of Biological Chemistry·Álvaro Gutiérrez-UzquizaAlmudena Porras
Mar 24, 2012·Biochimica Et Biophysica Acta·Fausto J RodriguezPanos Z Anastasiadis
Apr 3, 2012·Journal of Cell Science·Wenting Shih, Soichiro Yamada

❮ Previous
Next ❯

Citations

Feb 27, 2020·Cell Proliferation·Zeng YeXiaowu Xu
Aug 18, 2020·Autophagy·Xin ChenDaolin Tang
Dec 4, 2020·Cell Research·Daolin TangGuido Kroemer
Jan 31, 2021·Nature Reviews. Clinical Oncology·Xin ChenDaolin Tang
Apr 2, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Haiyan WangYongguang Tao
Apr 30, 2021·Biochemical Pharmacology·Guangxiang XuLianxiang Luo
Mar 30, 2020·Cell Chemical Biology·David A Armenta, Scott J Dixon
Sep 2, 2021·Cell Death and Differentiation·Xin ChenDaolin Tang
Oct 21, 2021·The FEBS Journal·Salimata Bagayoko, Etienne Meunier

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
FACS
flow cytometry
electrophoresis
fluorescence microscopy
confocal microscopy

Software Mentioned

ZEN2009
BD ModFit
Comet IV

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

© 2022 Meta ULC. All rights reserved