Cell-cell electrofusion: optimization of electric field amplitude and hypotonic treatment for mouse melanoma (B16-F1) and Chinese Hamster ovary (CHO) cells.

The Journal of Membrane Biology
Marko UsajMasa Kanduser

Abstract

Efficient electroporation of cells in physical contact induces cell fusion, and this process is known as electrofusion. It has been shown that appropriate hypotonic treatment of cells before the application of electric pulses can cause a significant increase in electrofusion efficiency. First, the amplitudes of the electric field were determined spectrofluorometrically, where sufficient permeabilization in hypotonic buffer occurred for B16-F1 and CHO cells. In further electrofusion experiments 14 +/- 4% of fused cells for B16-F1 and 6 +/- 1% for CHO was achieved. These electrofusion efficiencies, determined by double staining and fluorescence microcopy, are comparable to those of other published studies. It was also confirmed that successful electroporation does not necessarily guarantee high electrofusion efficiency due to biological factors involved in the electrofusion process. Furthermore, not only the extension of electrofusion but also cell survival depends on the cell line used. Further studies are needed to improve overall cell survival after electroporation in hypotonic buffer, which was significantly reduced, especially for B16-F1 cells. Another contribution of this report is the description of a simple modification o...Continue Reading

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Citations

Aug 9, 2012·The Journal of Membrane Biology·Ulrich TerpitzVladimir L Sukhorukov
Aug 28, 2012·The Journal of Membrane Biology·Damijan Miklavčič
Jul 12, 2014·Bioelectrochemistry·Feriel Siham HamdiBruno Le Pioufle
Jul 11, 2014·Expert Opinion on Drug Delivery·Maša Kandušer, Marko Ušaj
Oct 8, 2014·The Journal of Membrane Biology·Samo Mahnič-KalamizaDamijan Miklavčič
Jun 20, 2013·The Journal of Membrane Biology·Denis PavlihaDamijan Miklavčič
Mar 23, 2015·The Journal of Membrane Biology·P HenriJustin Teissié
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Mar 1, 2018·Scientific Reports·Rogier M SchoemanAlbert van den Berg
Jun 22, 2013·The Journal of Experimental Biology·Harunobu TsugiyamaYoshiaki Iwadate

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