Cell-cell interaction-dependent regulation of N-acetylglucosaminyltransferase III and the bisected N-glycans in GE11 epithelial cells. Involvement of E-cadherin-mediated cell adhesion.

The Journal of Biological Chemistry
Junko IijimaJianguo Gu

Abstract

Changes in oligosaccharide structures are associated with numerous physiological and pathological events. In this study, the effects of cell-cell interactions on N-linked oligosaccharides (N-glycans) were investigated in GE11 epithelial cells. N-glycans were purified from whole cell lysates by hydrazinolysis and then detected by high performance liquid chromatography and mass spectrometry. Interestingly, the population of the bisecting GlcNAc-containing N-glycans, the formation of which is catalyzed by N-acetylglucosaminyltransferase III (GnT-III), was substantially increased in cells cultured under dense conditions compared with those cultured under sparse conditions. The expression levels and activities of GnT-III but not other glycosyltransferases, such as GnT-V and alpha1,6-fucosyltransferase, were also consistently increased in these cells. However, this was not observed in mouse embryonic fibroblasts or MDA-MB231 cells, in which E-cadherin is deficient. In contrast, perturbation of E-cadherin-mediated adhesion by treatment with EDTA or a neutralizing anti-E-cadherin antibody abolished the up-regulation of expression of GnT-III. Furthermore, we observed the significant increase in GnT-III activity under dense growth condit...Continue Reading

References

Jan 1, 1990·Annual Review of Biochemistry·M Takeichi
Sep 1, 1983·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·H SchachterG Vella
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·M YoshimuraN Taniguchi
Feb 1, 1995·Biochemical Society Transactions·R A Dwek
Jun 7, 1996·The Journal of Biological Chemistry·M YoshimuraN Taniguchi
Oct 6, 1997·The EMBO Journal·M W RenshawM A Schwartz
Jun 22, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J W DennisC E Warren
Mar 4, 2000·Nature Medicine·M GranovskyJ W Dennis
Jun 26, 2001·Glycoconjugate Journal·A Kobata
Jul 12, 2001·The Journal of Biological Chemistry·O ZelphatiJ Corbeil
Jul 13, 2001·Current Opinion in Genetics & Development·A Locascio, M A Nieto
Jul 23, 2002·Nature Cell Biology·Egle AvizienyteMargaret C Frame
Nov 6, 2002·Biochimica Et Biophysica Acta·Minoru Fukuda
Nov 6, 2002·Biochimica Et Biophysica Acta·James W DennisMichael Demetriou
Apr 5, 2003·Annual Review of Biochemistry·John B Lowe, Jamey D Marth
Oct 15, 2003·The Journal of Cell Biology·Masaki ShigetaKiyotoshi Sekiguchi
Dec 4, 2003·Current Opinion in Cell Biology·Jean Paul Thiery
Mar 6, 2004·Science·W James Nelson, Roel Nusse
May 22, 2004·Annals of the New York Academy of Sciences·Rachel B HazanKimita Suyama
Sep 3, 2004·Current Biology : CB·Ann E Vernon, Carole LaBonne
Oct 7, 2004·Glycoconjugate Journal·Jianguo Gu, Naoyuki Taniguchi
Oct 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Xiangchun WangNaoyuki Taniguchi

❮ Previous
Next ❯

Citations

May 9, 2012·Glycoconjugate Journal·Jianguo GuYuguang Du
Nov 27, 2009·The Journal of Biological Chemistry·Yoshinobu KariyaJianguo Gu
Jan 27, 2012·American Journal of Physiology. Cell Physiology·Olga VaginGeorge Sachs
Apr 6, 2007·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Jianguo Gu
Oct 12, 2010·Cancer Chemotherapy and Pharmacology·Julio Cesar Madureira de Freitas JuniorJosé Andrés Morgado-Díaz
Nov 26, 2010·Cellular and Molecular Life Sciences : CMLS·Salomé S PinhoCelso A Reis
Jan 23, 2009·Biochemical and Biophysical Research Communications·Salomé S PinhoCelso A Reis
May 22, 2008·Cancer Science·Yan-Yang ZhaoNaoyuki Taniguchi
Apr 4, 2008·The FEBS Journal·Yanyang ZhaoNaoyuki Taniguchi
May 4, 2012·Journal of Cellular Biochemistry·Julio Cesar Madureira de-Freitas-JuniorJosé Andrés Morgado-Díaz
Jan 30, 2014·Reproduction in Domestic Animals = Zuchthygiene·P E FernándezC G Barbeito
May 23, 2012·Biochimica Et Biophysica Acta·Joana ParedesRaquel Seruca
Mar 6, 2009·Cell Adhesion & Migration·Jianguo Gu, Naoyuki Taniguchi
May 3, 2016·Biomolecules·Yasuhiko Kizuka, Naoyuki Taniguchi
Aug 28, 2016·Biochimica Et Biophysica Acta. General Subjects·Dorota Hoja-ŁukowiczMonika Bubka
Aug 21, 2014·Bioorganic & Medicinal Chemistry Letters·Shinya HanashimaYoshiki Yamaguchi
Feb 28, 2009·The Journal of Organic Chemistry·Guangfa WangYingxia Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.