Cell-cell interactions in the process of differentiation of thyroid epithelial cells into follicles: a study by microinjection and fluorescence microscopy on in vitro reconstituted thyroid follicles

Journal of Cellular Physiology
Y Munari-SilemB Rousset

Abstract

Thyroid cells, cultured in the presence of thyroid stimulating hormone, reorganized within 36-48 hr into follicular structures, the in vitro reconstituted thyroid follicles or RTF. By microinjection of fluorescent probes either into the neoformed intrafollicular lumen (IL) or into cells forming the follicles, we have studied the development and some functional properties of cell-cell contacts involved in a) the formation of the thyroid follicular lumen and b) the communication between thyrocytes within the follicle. The probes were compounds of either low (Lucifer Yellow: LY) or high molecular weight (Dextran labeled with fluorescein: FITC-Dextran and Cascade Blue conjugated to bovine serum albumin: CB-BSA). LY microinjected into IL of 2-9-day-old RTF was seen to label circular spaces with a diameter ranging from 10 to 100 microns. The cells delimiting the IL remained unlabeled. The fluorescent dye remained concentrated in IL for up to 24 hr. FITC-Dextran or CB-BSA microinjected into IL behaved as LY; the probes were restrained into the lumen. A 2 hr incubation of RTF with iodide induced alterations of the structure of IL; an effect mediated by an organic form of actively trapped iodide. A 15-30 min incubation of RTF in a low C...Continue Reading

References

Jul 21, 1976·Biochimica Et Biophysica Acta·B RoussetR Mornex
May 1, 1978·Endocrinology·A HaeberliS H Wollman
Aug 1, 1988·Journal of Biochemical and Biophysical Methods·W Ansorge, R Pepperkok
Mar 1, 1987·Molecular and Cellular Biochemistry·C DurrieuB Rousset
Jun 15, 1987·European Journal of Biochemistry·Y Malthiéry, S Lissitzky
Mar 1, 1986·Molecular and Cellular Endocrinology·Y Munari-SilemB Rousset
Jun 1, 1971·The Journal of Clinical Endocrinology and Metabolism·W L RobisonD Davis
Dec 1, 1983·Archives of Biochemistry and Biophysics·S FormisanoG Palumbo
Jan 1, 1982·Vitamins and Hormones·J Nunez, J Pommier
Dec 1, 1980·The Journal of Cell Biology·A Martinez-PalomoM Cereijido
Apr 1, 1981·Molecular and Cellular Endocrinology·L E Ericson
Nov 1, 1981·Molecular and Cellular Endocrinology·R Ekholm

❮ Previous
Next ❯

Citations

Aug 1, 1996·Journal of Bioenergetics and Biomembranes·P Meda
May 17, 1996·Molecular and Cellular Endocrinology·S Selmi-Ruby, B Rousset
Aug 31, 2011·Thyroid : Official Journal of the American Thyroid Association·Claudia DominguezJean-Louis Kraimps
Nov 18, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·P PocarS Hombach-Klonisch
May 1, 1995·Annals of Saudi Medicine·N R Farid
Jan 1, 1997·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·T Amphoux-FazekasN Samih

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.