Cell compatibility of fibrin sealants: in vitro study with cells involved in soft tissue repair

Journal of Biomaterials Applications
Diana MacasevMichaela Bittner

Abstract

Fibrin sealants can be used to support tissue regeneration or as vehicles for delivery of cells in tissue engineering. Differences in the composition of fibrin sealants, however, could determine the success of such applications. The results presented in this article show clear differences between Fibrin sealant A (FS A) clots and Fibrin sealant B (FS B) clots with respect to their compatibility with primary human cells involved in soft tissue repair. FS A clots, which are characterized by a physiological coarse fibrin structure, promoted attachment, spreading, and proliferation of keratinocytes, fibroblasts, and endothelial cells. In contrast, FS B clots displaying a fine to medium clot structure failed to support spreading of all three cell types. Adhesion of keratinocytes was decreased on FS B clots compared to FS A clots after 3 h incubation, whereas number of attached fibroblasts and endothelial cells was initially comparable between the two fibrin sealants. However, all three cell types proliferated on FS A clots but no sustained proliferation was detected on FS B clots. We further demonstrate that the observed differences between FS A and B clots are partly based upon 1 M sodium chloride extractable constituents, like thr...Continue Reading

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Citations

Sep 19, 2014·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Alfred GugerellRainer Mittermayr
Jul 22, 2014·Journal of Tissue Engineering and Regenerative Medicine·Susanne WolbankHeinz Redl
Aug 4, 2015·BioMed Research International·Johanna KoberMaike Keck
Dec 6, 2018·Journal of Tissue Engineering and Regenerative Medicine·Christian KrugElias Volkmer
Feb 24, 2012·Journal of Biomedical Materials Research. Part a·Alfred GugerellWaltraud Pasteiner
Jul 26, 2015·Journal of Biomaterials Applications·Lei HouXingyu Liu

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