PMID: 9371614Nov 26, 1997Paper

Cell culture adaptation of Puumala hantavirus changes the infectivity for its natural reservoir, Clethrionomys glareolus, and leads to accumulation of mutants with altered genomic RNA S segment

Journal of Virology
Ake LundkvistAlexander Plyusnin

Abstract

This paper reports the establishment of a model for hantavirus host adaptation. Wild-type (wt) (bank vole-passaged) and Vero E6 cell-cultured variants of Puumala virus strain Kazan were analyzed for their virologic and genetic properties. The wt variant was well adapted for reproduction in bank voles but not in cell culture, while the Vero E6 strains replicated to much higher efficiency in cell culture but did not reproducibly infect bank voles. Comparison of the consensus sequences of the respective viral genomes revealed no differences in the coding region of the S gene. However, the noncoding regions of the S gene were found to be different at positions 26 and 1577. In one additional and independent adaptation experiment, all analyzed cDNA clones from the Vero E6-adapted variant were found to carry substitutions at position 1580 of the S segment, just 3 nucleotides downstream of the mutation observed in the first adaptation. No differences were found in the consensus sequences of the entire M segments from the wt and the Vero E6-adapted variants. The results indicated different impacts of the S and the M genomic segments for the adaptation process and selective advantages for the variants that carried altered noncoding seque...Continue Reading

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Citations

Mar 8, 2008·Emerging Infectious Diseases·Lisa PetterssonMagnus Evander
Dec 2, 2008·Journal of Medical Virology·Jonas HardestamJonas Klingström
Nov 6, 2009·Journal of Virology·Jonas Schmidt-ChanasitRainer G Ulrich
Apr 25, 2020·Virus Genes·Florian BinderRainer G Ulrich
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Aug 22, 2008·PloS One·Tarja SironenAlexander Plyusnin
Apr 19, 2008·The Journal of General Virology·Tarja SironenAlexander Plyusnin
May 9, 2008·Reviews in Medical Virology·Antti VaheriAlexander Plyusnin
May 12, 2010·Vector Borne and Zoonotic Diseases·Adrian N BillingsCharles F Fulhorst
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Jan 5, 2010·The Journal of Veterinary Medical Science·Hiroaki KariwaIkuo Takashima
Apr 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·David SafronetzHeinz Feldmann
Jan 31, 2019·Scientific Reports·Carles Solà-RieraJonas Klingström
Jun 27, 2006·Scandinavian Journal of Infectious Diseases·Angelina PlyusninaAlexander Plyusnin
Aug 6, 2008·Emerging Infectious Diseases·Jonas HardestamAke Lundkvist
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