Cell culture and PCR determination of poliovirus inactivation by disinfectants.

Applied and Environmental Microbiology
J F MaC P Gerba

Abstract

Inactivation of poliovirus type 1 by 1 N HCl, 1 N NaOH, 0.5 and 1.0 mg of free chlorine per liter, and UV light was compared by using cell culture and seminested PCR (30 cycles of reverse transcriptase-PCR plus 30 cycles of seminested PCR). A minimum contact time of 45 min with HCl, 3 min with NaOH, 3 and 6 min with 1.0 and 0.5 mg of free chlorine per liter, respectively, was required to render 1.64 x 10(2) PFU of poliovirus type 1 per ml undetectable by seminested PCR. In cell culture, a minimum contact time of 5 min to HCl, 30 s to NaOH, and 1 min to either chlorine concentration was required to render the viruses undetectable by the plaque assay method. No correlation was observed between results by PCR and cell culture when viruses were exposed to UV light. These data suggest that inactivated virus with intact nucleic acid sequences can be detected by PCR.

References

May 1, 1993·Applied and Environmental Microbiology·M AbbaszadeganI L Pepper
Jun 1, 1994·Applied and Environmental Microbiology·J F MaC P Gerba
Dec 1, 1993·PCR Methods and Applications·R W Cone, M R Fairfax
Mar 1, 1994·Applied and Environmental Microbiology·T M StraubC P Gerba
Oct 1, 1993·Applied and Environmental Microbiology·J GraffB Flehmig
Oct 1, 1993·Applied and Environmental Microbiology·K L JosephsonI L Pepper
Jan 1, 1993·Reviews of Environmental Contamination and Toxicology·T M StraubC P Gerba

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Citations

Jul 4, 2003·Applied and Environmental Microbiology·Benoît GassilloudChristophe Gantzer
Feb 13, 2014·Scientific Reports·Veena V UnnithanGeoffrey B Smith
May 2, 2000·Applied and Environmental Microbiology·F BlackmerI L Pepper
Sep 27, 2002·Applied and Environmental Microbiology·Jun Wen LiFu Huan Chao
Oct 17, 2006·Applied and Environmental Microbiology·Julien Simonet, Christophe Gantzer
Jan 7, 2003·Applied and Environmental Microbiology·Suphachai Nuanualsuwan, Dean O Cliver
Dec 23, 2006·Risk Analysis : an Official Publication of the Society for Risk Analysis·Walter DowdleChris Wolff
Nov 18, 2008·Applied and Environmental Microbiology·Roberto A RodríguezCharles P Gerba
May 8, 2008·Avian Diseases·M E LombardiE R Benson
Mar 23, 2010·Applied and Environmental Microbiology·Martin A PageBenito J Mariñas
Mar 24, 2006·Journal of Applied Microbiology·J Simonet, C Gantzer
Aug 4, 2001·Environmental Health Perspectives·S E HenricksonP R Epstein
Oct 23, 1997·Clinical Microbiology Reviews·W A Rutala, D J Weber

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