Cell culture propagation and biochemical analysis of the Ljungan virus prototype strain

Biochemical and Biophysical Research Communications
E Susanne JohanssonA M Lindberg

Abstract

Ljungan virus (LV) is proposed as a potentially important rodent harbored viral human pathogen. Little is known about the biophysical nature of the virus and despite being molecularly characterized, progress in epidemiological and basic biological studies of LV has been hampered by the lack of a robust and reliable cell culture propagation system. Here we report the first description of an efficient lytic multi-cycle cell culture propagation of the LV prototype strain (87-012). Biophysical analysis of gradient purified LV virions generated by this system identified mature infectious virions to possess a sedimentation coefficient of 160S and in agreement with previous molecular prediction, polyprotein analysis suggests that the native virion is composed of only three major structural proteins. The nucleotide composition of the complete genome of the LV cell culture adapted virus was determined and compared to that of the parental prototype LV. Numerous mutations were observed scattered throughout the viral genome and particularly in VP1. The development of this cell culture system for LV should open new avenues in the study of LV biology, structure, pathogenesis, and prevalence of natural infection in the wider community.

References

Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J B FlaneganD Baltimore
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·Timo HyypiäG Stanway
Nov 1, 1991·The Journal of General Virology·Darren R Shafren, G A Tannock
Jun 1, 1990·Journal of the American College of Cardiology·S TracyN Chapman
Apr 1, 1997·The Journal of General Virology·M D Ryan, M Flint
Nov 20, 1998·The Journal of General Virology·F GhaziG Stanway
May 15, 2003·International Journal of Experimental Diabesity Research·B NiklassonAke Lernmark

Citations

Dec 24, 2011·PloS One·Malin StoltzJonas Klingström
May 15, 2012·Journal of Virological Methods·René KalliesMatthias Niedrig
Jul 16, 2013·Journal of Medical Virology·Anne J JääskeläinenOlli Vapalahti
Apr 12, 2008·Journal of Virological Methods·Conny TolfA M Lindberg
Aug 17, 2005·Journal of Virological Methods·Per AnderssonA M Lindberg
Oct 9, 2015·Nature Communications·Ling ZhuDavid I Stuart

Related Concepts

Cercopithecus tantalus
Cricetus
Macaca mulatta
Family Picornaviridae
Vero Cells
Virion
Virus Cultivation
Virus Replication
Chinese Hamster Ovary Cell
Viral Genome

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Archaeal RNA Polymerase

Archaeal RNA polymerases are most similar to eukaryotic RNA polymerase II but require the support of only two archaeal general transcription factors, TBP (TATA-box binding protein) and TFB (archaeal homologue of the eukaryotic general transcription factor TFIIB) to initiate basal transcription. Here is the latest research on archaeal RNA polymerases.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.