Cell cycle- and chromatin binding state-dependent phosphorylation of human MCM heterohexameric complexes. A role for cdc2 kinase.

The Journal of Biological Chemistry
M FujitaM Inagaki

Abstract

The mammalian MCM protein family, presently with six members, exists in the nuclei in two forms, chromatin-bound and unbound. The former dissociates from chromatin with progression through the S phase. Recently, we have established a procedure to isolate chromatin-bound and unbound complexes containing all six human MCM (hMCM) proteins by immunoprecipitation. In the present study, we applied this procedure to HeLa cells synchronized in each of the G1, S, and G2/M phases and could detect hMCM heterohexameric complexes in all three. In addition, depending on the cell cycle and the state of chromatin association, hMCM2 and 4 in the complexes were found to variously change their phosphorylation states. Concentrating attention on G2/M phase hyperphosphorylation, we found hMCM2 and 4 in the complexes to be good substrates for cdc2/cyclin B in vitro. Furthermore, when cdc2 kinase was inactivated in temperature-sensitive mutant murine FT210 cells, the G2/M hyperphosphorylation of the murine MCM2 and MCM4 and release of the MCMs from chromatin in the G2 phase were severely impaired. Taken together, the data suggest that the six mammalian MCM proteins function and undergo cell cycle-dependent regulation as heterohexameric complexes and t...Continue Reading

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y ChenB K Tye
Jun 1, 1992·The Journal of Cell Biology·J R HamaguchiE M Bradbury
Jun 1, 1991·Genes & Development·K M HennessyD Botstein
Jun 15, 1995·European Journal of Biochemistry·C MusahlR Knippers
Jul 15, 1995·Genes & Development·J E TreismanG M Rubin
Mar 1, 1995·European Journal of Biochemistry·R BurkhartR Knippers
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·S Dalton, L Whitbread
Jun 1, 1995·The Journal of Cell Biology·I T TodorovS E Kearsey
Oct 1, 1994·Molecular Biology of the Cell·K TakahashiM Yanagida
Feb 15, 1996·Biochemical and Biophysical Research Communications·M FujitaM Ishibashi
Feb 23, 1996·The Journal of Biological Chemistry·M FujitaM Ishibashi
Feb 1, 1996·Molecular Biology of the Cell·T T SuP H O'Farrell
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·P RomanowskiR A Laskey
Mar 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S E KearseyI T Todorov
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·M HendricksonJ Gautier
Nov 1, 1996·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·H KimuraK Sugimoto
Apr 18, 1997·The Journal of Biological Chemistry·M FujitaM Ishibashi
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·S DonovanJ F Diffley
Sep 26, 1997·The Journal of Biological Chemistry·Y Ishimi

❮ Previous
Next ❯

Citations

May 15, 2001·International Journal of Cancer. Journal International Du Cancer·W El-RifaiS Knuutila
Feb 22, 2002·Journal of Cellular Physiology·Hisao Masai, Ken-Ichi Arai
Mar 24, 2004·Journal of Structural Biology·Yukio IshimiKouichi Yamada
Jan 14, 2003·EMBO Reports·Lüder WiebuschChristian Hagemeier
Feb 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yuko KatsunoMakoto Nakanishi
May 22, 2010·The Journal of Biological Chemistry·Hong Shen, Carl G Maki
Aug 12, 2008·The Journal of Biological Chemistry·Lova RakotomalalaOurania Andrisani
Nov 19, 2008·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Megan L ShoemakerThomas R Hansen
Jun 15, 2004·Molecular Biology of the Cell·Yoshiki YamadaHisao Masukata
Dec 29, 2007·Molecular Biology of the Cell·Nozomi SugimotoMasatoshi Fujita
Nov 16, 2012·Journal of Virology·Xueli FanJason J Chen
Feb 26, 2005·Journal of Virology·Tohru DaikokuTatsuya Tsurumi
Nov 17, 2010·Molecular and Cellular Biology·Taku ChibazakuraJames M Roberts
Apr 25, 2000·Molecular and Cellular Biology·I PereverzevaM Coué
Mar 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Susan L Forsburg
Jun 5, 2002·Annual Review of Biochemistry·Stephen P Bell, Anindya Dutta
Feb 17, 2010·Journal of Biomedical Science·Bertrand Chin-Ming TanSheng-Chung Lee
Jun 2, 2009·Frontiers in Bioscience (Landmark Edition)·Jeanette Gowen Cook
Jul 19, 2013·Journal of Biochemistry·Mariko Moritani, Yukio Ishimi
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·David CortezStephen J Elledge
Sep 11, 2007·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Arjen KoppenLinda J Valentijn
Dec 5, 2006·Journal of Molecular Biology·Anthony L GotterBeverly S Emanuel
Mar 8, 2006·The FEBS Journal·Yuki Komamura-KohnoYukio Ishimi
Aug 31, 2000·Annual Review of Biochemistry·T J Kelly, G W Brown
Sep 24, 2004·Current Biology : CB·John F X Diffley
Apr 20, 2005·Oncogene·David Y Takeda, Anindya Dutta
Sep 6, 2018·Journal of Biochemistry·Xuan Wang, Yukio Ishimi
Sep 27, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Thomas D FriedrichJohn M Lehman
Oct 19, 2006·The Journal of Biological Chemistry·Hisao MasaiKen-Ichi Arai
Apr 5, 2000·The Journal of Biological Chemistry·Y IshimiM Kitagawa
Feb 29, 2000·The Journal of Biological Chemistry·Y ArataJ Y Kato
Jul 17, 2001·The Journal of Biological Chemistry·Y Ishimi, Y Komamura-Kohno
Apr 26, 2003·The Journal of Biological Chemistry·Yukio IshimiMakoto Nakanishi
Mar 3, 2004·The Journal of Biological Chemistry·Nozomi SugimotoMasatoshi Fujita
Jan 22, 2005·The Journal of Biological Chemistry·Hiroko Fujii-YamamotoHisao Masai

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.