Cell cycle analysis and detection of proliferative cell nuclear antigen of the endometrium after hormone replacement therapy

Maturitas
S N ChowC H Chien

Abstract

To understand the effect of sequential combined hormone replacement therapy (HRT) on the postmenopausal endometrium. Sonographic endometrial thickness, endometrial histopathology, flow cytometric cell cycle analysis and the level of proliferative cell nuclear antigen (PCNA) were studied. One hundred and thirty-eight postmenopausal women were enrolled in this study. Among which, 97 women had their endometrium being adequately obtained; the most frequent type of histopathology was normal endometrium (91.8%). Endometrial hyperplasia was found in seven patients (7.2%), including typical simple hyperplasia (n=1, 1%), focal simple hyperplasia (n=5, 5.2%) and complex hyperplasia without atypia (n=1, 1%). The proliferative fractions (PF; S plus G2-M phase) of cells from normal and hyperplastic endometrium of menopausal women after HRT were 8.18 and 8.95%, respectively, which were lower than those from 29 premenopausal women without HRT. The level of PCNA of normal and hyperplastic endometrium in postmenopausal women after HRT was about 80 and 84%, respectively, of that from premenopausal endometrium. Our study showed the PF of the cell cycle and the level of PCNA were not increased in the menopausal endometrium under HRT as compared to...Continue Reading

References

Jan 1, 1976·Canadian Journal of Biochemistry·G JackowskiC C Liew
Dec 4, 1975·The New England Journal of Medicine·H K Ziel, W D Finkle
Jun 3, 1976·The New England Journal of Medicine·T M MackS E Brown
Jan 4, 1979·The New England Journal of Medicine·C M AntunesR Garcia
Mar 1, 1979·American Journal of Obstetrics and Gynecology·C B HammondR T Parker
Dec 4, 1975·The New England Journal of Medicine·D C SmithW L Herrmann
Jan 1, 1986·Acta Obstetricia Et Gynecologica Scandinavica. Supplement·M I Whitehead
Jan 1, 1987·American Journal of Obstetrics and Gynecology·B J RiisC Christiansen
Oct 1, 1987·The Journal of Clinical Endocrinology and Metabolism·L Nilas, C Christiansen
Dec 1, 1981·The Journal of Experimental Medicine·Y TakasakiE M Tan
Aug 28, 1980·The New England Journal of Medicine·S ShapiroR C Knapp
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F DolbeareJ W Gray
Jan 1, 1995·Gynecologic and Obstetric Investigation·H Ishihara, H Itamochi
Mar 1, 1996·Maturitas·B S Hulka, O Meirik
Dec 1, 1995·European Journal of Obstetrics, Gynecology, and Reproductive Biology·I NociG Taddei
May 23, 1998·Molecular Human Reproduction·Y YokoyamaT Tamaya
Aug 24, 2000·The New England Journal of Medicine·D M HerringtonD Waters

Related Concepts

Monoclonal Antibodies
Cell Cycle
Drug Administration Schedule
SDS-PAGE
Endometrium
Dagynil
Flow Cytometry
Tomography, Ultrasonic
Western Blot
Nested Case-Control Studies

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.