Cell cycle analysis of Epstein-Barr virus-infected cells following treatment with lytic cycle-inducing agents

Journal of Virology
A RodriguezE Flemington

Abstract

While Epstein-Barr virus (EBV) latency-associated gene expression is associated with cell cycle progression, the relationship between the EBV lytic program and the cell cycle is less clear. Using four different EBV lytic induction systems, we address the relationship between lytic cycle activation and the cell cycle. In three of these systems, G0 or G1 cell growth arrest signaling is observed prior to detection of the EBV immediate-early gene product Zta. In tetradecanoyl phorbol acetate-treated P3HR1 cultures and in 5-iodo-2'-deoxyuridine-treated NPC-KT cultures, cell cycle analysis of Zta-expressing cell populations showed a significant G1 bias during the early stages of lytic cycle progression. In contrast, treatment of the cell line Akata with anti-immunoglobulin (Ig) results in rapid induction of immediate-early gene expression, and accordingly, activation of the immediate-early gene product Zta precedes significant anti-Ig-induced cell cycle effects. Nevertheless, cell cycle analysis of the Zta-expressing population following anti-Ig treatment shows a bias for cells in G1, indicating that anti-Ig-mediated induction of Zta occurs more efficiently in cells traversing G1. Last, although 5-azacytidine treatment of Rael cells ...Continue Reading

References

Oct 1, 1992·Seminars in Cancer Biology·L S Young, M Rowe
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J BeckerH Wolf
Sep 1, 1984·Journal of Virology·H WolfE Wilmes
Nov 14, 1997·Trends in Microbiology·S H SpeckE Flemington
Dec 24, 1997·The Journal of General Virology·A HossainC Jones
Aug 19, 1999·Journal of the National Cancer Institute·M BonnetI Joab

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Citations

May 28, 2005·Journal of Virology·Amy L Adamson
Jan 17, 2009·Cell Division·Adam J Hume, Robert F Kalejta
Mar 25, 2011·Herpesviridae·Rhiannon R Penkert, Robert F Kalejta
Jun 15, 2007·PLoS Pathogens·Arnaud ChêneMaria Teresa Bejarano
May 27, 2015·Viruses·Robert Hollingworth, Roger J Grand
Jun 23, 2009·Free Radical Biology & Medicine·Helen I Osipova-GoldbergJosef M Pfeilschifter
Dec 5, 2015·Acta Oto-laryngologica·Arunkumar AnandharajCherie-Ann O Nathan
Oct 10, 2009·International Journal of Cancer. Journal International Du Cancer·K F Hui, Alan K S Chiang
Feb 19, 2003·Biochemical and Biophysical Research Communications·Li-Kwan ChangShih-Tung Liu
Aug 26, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Ju-Hong JiangYi-Xin Zeng
Dec 3, 2014·Current Opinion in Virology·Niza FrenkelHaim Zeigerman
Jun 10, 2015·Viruses·Robert HollingworthRoger J Grand
Feb 7, 2014·Journal of Virology·Patrick PaladinoLori Frappier
Apr 20, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dan WangCan Guo
Jun 6, 2020·The Journal of General Virology·Robert HollingworthRoger J Grand
Mar 16, 2019·Frontiers in Oncology·Kwai Fung HuiAlan Kwok Shing Chiang
Apr 2, 2020·Seminars in Immunopathology·Alexander Buschle, Wolfgang Hammerschmidt
Aug 28, 2020·Journal of Virology·Mengmeng ZhaoZhen Lin

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