PMID: 11316567Apr 24, 2001

Cell cycle and growth response of CHO cells to X-irradiation: threshold-free repair at low doses

International Journal of Radiation Oncology, Biology, Physics
D BartkowiakE M Röttinger

Abstract

To test the hypothesis of a threshold for induced repair of DNA damage (IR) and, secondarily, of hyperradiosensitivity (HRS) to low-dose X-irradiation. Exponentially growing Chinese hamster ovary cells (CHO) were X-irradiated with doses from 0.2 to 8 Gy. Survival data were established by conventional colony-forming assay and flow-cytometric population counting. The early cell cycle response to radiation was studied based on DNA-profiles and bromodeoxyuridine pulse-labeling experiments. Colony-forming data were consistent with HRS. However, these data were of low statistic significance. Population counting provided highly reproducible survival curves that were in perfect accord with the linear-quadratic (LQ) model. The dominant cell cycle reaction was a dose-dependent delay of G2 M and late S-phase. There was no evidence for a threshold of IR and for low-dose HRS in X-irradiated CHO cells. It is suggested that DNA damage repair activity is constitutively expressed during S-phase and is additionally induced in a dose-dependent and threshold-free manner in late S-phase and G2. The resulting survival is precisely described by the LQ model.

References

Aug 1, 1989·The British Journal of Radiology·Jack F Fowler
Mar 1, 1989·International Journal of Radiation Oncology, Biology, Physics·H P Beck-BornholdtF Würschmidt
Mar 1, 1989·Mutation Research·N S Rudolph, S A Latt
Jul 1, 1986·International Journal of Radiation Oncology, Biology, Physics·L D SkarsgardB Palcic
Dec 1, 1984·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·J DeaconG G Steel
Jan 1, 1993·International Journal of Radiation Oncology, Biology, Physics·S H KimJ H Kim
Aug 23, 1996·The Journal of Biological Chemistry·H BeamishM F Lavin
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·T T PUCKS Rasumussen
Mar 1, 1997·International Journal of Radiation Oncology, Biology, Physics·Jack F Fowler, E F Van Limbergen
May 11, 1999·DNA and Cell Biology·B S TzangY C Liu
Jun 5, 1999·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·D BartkowiakE M Röttinger
Sep 7, 1999·International Journal of Radiation Oncology, Biology, Physics·L G SmithE J Hall
Dec 28, 1999·Molecular and Cellular Biology·R M TaylorK W Caldecott

Citations

Jun 28, 2003·International Journal of Radiation Oncology, Biology, Physics·Jack F FowlerDavid J Brenner
Jul 2, 2014·Cancer Letters·Lynn M MartinLaure Marignol
Apr 18, 2002·International Journal of Radiation Oncology, Biology, Physics·Nicole Boehringer-WyssAbdelkarim S Allal
May 23, 2013·Cancer Letters·Lynn M MartinLaure Marignol
Jun 16, 2010·International Journal of Radiation Biology·Shumei MaXiaodong Liu
Apr 4, 2008·International Journal of Radiation Biology·Sarah KoenigHans Christiansen
Apr 1, 2008·International Journal of Radiation Oncology, Biology, Physics·B Marples, S J Collis
Jan 24, 2007·International Journal of Radiation Oncology, Biology, Physics·L M GarciaG P Raaphorst
Jul 6, 2004·International Journal of Radiation Oncology, Biology, Physics·Timothy M Pawlik, Khandan Keyomarsi
May 2, 2013·International Journal of Radiation Biology·Charles ThomasNicolas Foray
May 26, 2006·Physics in Medicine and Biology·L M GarciaG P Raaphorst

Related Concepts

Cell Cycle
Cell Division Phases
Cell Survival
Colony-Forming Units Assay
DNA, Double-Stranded
Genotoxic Stress
Base Excision Repair
Dose-Response Relationship, Radiation
Cricetus
Radiation Tolerance

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.