PMID: 11316567Apr 24, 2001Paper

Cell cycle and growth response of CHO cells to X-irradiation: threshold-free repair at low doses

International Journal of Radiation Oncology, Biology, Physics
D BartkowiakE M Röttinger

Abstract

To test the hypothesis of a threshold for induced repair of DNA damage (IR) and, secondarily, of hyperradiosensitivity (HRS) to low-dose X-irradiation. Exponentially growing Chinese hamster ovary cells (CHO) were X-irradiated with doses from 0.2 to 8 Gy. Survival data were established by conventional colony-forming assay and flow-cytometric population counting. The early cell cycle response to radiation was studied based on DNA-profiles and bromodeoxyuridine pulse-labeling experiments. Colony-forming data were consistent with HRS. However, these data were of low statistic significance. Population counting provided highly reproducible survival curves that were in perfect accord with the linear-quadratic (LQ) model. The dominant cell cycle reaction was a dose-dependent delay of G2 M and late S-phase. There was no evidence for a threshold of IR and for low-dose HRS in X-irradiated CHO cells. It is suggested that DNA damage repair activity is constitutively expressed during S-phase and is additionally induced in a dose-dependent and threshold-free manner in late S-phase and G2. The resulting survival is precisely described by the LQ model.

References

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Citations

Jun 28, 2003·International Journal of Radiation Oncology, Biology, Physics·Jack F FowlerDavid J Brenner
Jul 2, 2014·Cancer Letters·Lynn M MartinLaure Marignol
Apr 18, 2002·International Journal of Radiation Oncology, Biology, Physics·Nicole Boehringer-WyssAbdelkarim S Allal
May 23, 2013·Cancer Letters·Lynn M MartinLaure Marignol
Jun 16, 2010·International Journal of Radiation Biology·Shumei MaXiaodong Liu
Apr 4, 2008·International Journal of Radiation Biology·Sarah KoenigHans Christiansen
Apr 1, 2008·International Journal of Radiation Oncology, Biology, Physics·Brian Marples, Spencer J Collis
Jan 24, 2007·International Journal of Radiation Oncology, Biology, Physics·Lourdes M GarciaGijsbert P Raaphorst
Jul 6, 2004·International Journal of Radiation Oncology, Biology, Physics·Timothy M Pawlik, Khandan Keyomarsi
May 2, 2013·International Journal of Radiation Biology·Charles ThomasNicolas Foray
May 26, 2006·Physics in Medicine and Biology·L M GarciaG P Raaphorst

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