Cell cycle and radiosensitivity of progeny of irradiated primary cultured human hepatocarcinoma cells

World Journal of Gastroenterology : WJG
Zhi-Zhong LiuJia-Long Huang

Abstract

To evaluate the change of growth characteristics and radiosensitivity of irradiated primary cultured human hepatocarcinoma cells. All tumor tissue samples were obtained from 39 hepatocarcinoma patients with a mean age of 49.6 years (range 22-76 years). We divided the samples into irradiated group and non-irradiated group and measured their plating efficiency (PE), population doubling time (PDT), radiosensitivity index SF2 and cell cycle. The PDT of primary culture of hepatocarcinoma cells was 91.0+/-6.6 h, PE was 12.0+/-1.4%, SF2 was 0.41+/-0.05%. The PDT of their irradiated progeny was 124.8+/-5.8 h, PE was 5.0+/-0.7%, SF2 was 0.65+/-0.09%. The primary cultured human hepatocarcinoma cells showed significant S reduction and G(2) arrest in a dose-dependent manner. The progeny of irradiated primary cultured hepatocarcinoma cells grew more slowly and its radiosensitivity increased. The progeny of irradiated primary cultured human hepatocarcinoma cells grows more slowly and its radiosensitivity increases.

References

Mar 30, 2001·The Journal of Biological Chemistry·B HuY Wang
Jul 11, 2002·British Journal of Cancer·J-L KuJ-G Park

Related Concepts

Cell Cycle
Cell Survival
Liver Carcinoma
Malignant Neoplasm of Liver
Radiation Tolerance
Tumor Cells, Cultured
Roentgen Rays

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